During aPCR reaction, what would be the function of Taq DNA polymerase? extends the primers into...

During aPCR reaction, what would be the function of Taq DNA polymerase?

extends the primers into full-length PCR product (i.e. copies the DNA)

unwinds dsDNA template

ensures DNA is negatively charged for gel electrophoresis

cuts DNA into fragments

assists primers in annealing to the template

Solutions

Expert Solution

extends the primers into full-length PCR product (i.e. copies the DNA)

Explanation: Taq polymerase can tolerate high temperature and can extend primer to synthesize new strand in 5' to 3' direction. Without primer this enzyme can not perform synthesis.

Explanation for wrong answers

unwinds dsDNA template

Explanation: Increasing temperature in PCR leads to the melting of double stranded DNA.

ensures DNA is negatively charged for gel electrophoresis

Explanation: Taq polymerase is the enzyme and it perform the function of polymerization. Presence of SDS in the reagents of electrophoresis gives uniform negative charge to the proteins.

cuts DNA into fragments

Explanation: This is the function of restriction enzymes.

assists primers in annealing to the template

Explanation: Decreasing melting temperature in PCR will lead to the annealing of the primers. Taq polymerase do not have any role in the annealing.

gladiator answered 1 year ago

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