Question

In: Biology

CSSR may require all of the following except__________ cis-acting elements in the target (i.e. the recipient)...

CSSR may require all of the following except__________

cis-acting elements in the target (i.e. the recipient) DNA

Rec A, B and C

a sequence specific DNA binding nuclease/DNA ligase

some architectural (non-enzymatic) proteins to aid the process
cis-acting elements in the donor DNA

Solutions

Expert Solution

Rec A, B and C

DNA strand with a certain degree of sequence homology will undergo site-specific recombination and here the site-specific recombinases (SSRs) will perform the rearrangements of DNA segments by recognizing it and will go and bind to the short DNA sequences, and then cleave the DNA backbone in order to exchange the two DNA helices which are involved and finally rejoin the DNA strands. The CSSR needs everything except Rec A, B and C as the process will occur independent of Rec proteins.


Related Solutions

Describe the difference between cis-acting and trans-acting elements. Also list whether the following elements are cis- or trans-acting:
Describe the difference between cis-acting and trans-acting elements. Also list whether the following elements are cis- or trans-acting: A) β-galactosidase B) Operator site C) LacI repressor protein D) Lactose E) Permease F) Promoter site
Describe the difference between cis-acting and trans-acting elements. Also list whether the following elements are cis-...
Describe the difference between cis-acting and trans-acting elements. Also list whether the following elements are cis- or trans-acting: A) Beta-galactosidase B) Operator site C) LacI repressor protein D) Lactose E) Permease F) Promoter site
Of all the cis-acting elements associated with a gene, which is the most important? Why?
Of all the cis-acting elements associated with a gene, which is the most important? Why?
Which of the following genes of the Lac operon encode cis-acting regulatory elements?
Which of the following genes of the Lac operon encode cis-acting regulatory elements?  Lacy  Lacl None of these answers is correct  LacA  Lacz
Which are characteristics of regulatory transcription factors? (select all that apply) a. Act at cis-acting elements...
Which are characteristics of regulatory transcription factors? (select all that apply) a. Act at cis-acting elements b. Activity modulated by protein-protein interactions c. Bind to promoter sequence d. Activity sometimes modulated by small effector molecules e. Regulate transcription of specific target genes f. Can act to repress or activate transcription g. Activity modulated by covalent modifications h. Necessary for basal level of transcription
Compare the cis acting elements of prokaryotic and eukaryotic transcriptional regulation.
Compare the cis acting elements of prokaryotic and eukaryotic transcriptional regulation.
How can cis- and trans- acting regulatory elements be distinguished?
How can cis- and trans- acting regulatory elements be distinguished?
For each of the following, indicate if it is cis-acting or trans-acting
For each of the following, indicate if it is cis-acting or trans-actingA regulatory gene that codes for a transcription factor _______________A promoter _______________An operator _________________An enhancer _________________
The necessary components for gene expression are often described as either cis-acting elements (“acting from the...
The necessary components for gene expression are often described as either cis-acting elements (“acting from the same molecule”) or trans-acting factors (“acting from a different molecule”). Cis-acting elements are typically sequences that are required for expression. Trans-acting factors are typically proteins that recognize and bind cis-acting sequences to control or facilitate gene expression. For each of the cis-acting elements below, Promotor and Start Codon,list a trans-acting factor involved in its recognition and describe the factor’s normal function
Consider the lac operon in E. coli. a. List the cis-acting elements and the trans-acting factors...
Consider the lac operon in E. coli. a. List the cis-acting elements and the trans-acting factors involved in the regulation of its expression. b. Indicate which Transacting factors interact with which cis-elements. c. Describe what the factors do when they are bound to their respective cis–elements. d. Indicate the inducer in this system and how it works.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT