CSSR may require all of the following except__________ cis-acting elements in the target (i.e. the recipient)...

CSSR may require all of the following except__________

cis-acting elements in the target (i.e. the recipient) DNA

Rec A, B and C

a sequence specific DNA binding nuclease/DNA ligase

some architectural (non-enzymatic) proteins to aid the process

cis-acting elements in the donor DNA

Solutions

Expert Solution

Rec A, B and C

DNA strand with a certain degree of sequence homology will undergo site-specific recombination and here the site-specific recombinases (SSRs) will perform the rearrangements of DNA segments by recognizing it and will go and bind to the short DNA sequences, and then cleave the DNA backbone in order to exchange the two DNA helices which are involved and finally rejoin the DNA strands. The CSSR needs everything except Rec A, B and C as the process will occur independent of Rec proteins.

gladiator answered 1 year ago

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