How can differential centrifugation be used to distinguishbetween ribosomal subunits of eukaryotes and prokaryotes?Specifically...

How can differential centrifugation be used to distinguish between ribosomal subunits of eukaryotes and prokaryotes?

Specifically 60s/40s and 50S/30S.

Expert Solution

Differential centrifugation creates a sort of gradient where in denser heavy particles settle at bottom and lighter counter parts stay in supernatent .... since there is difference in (S) swedberg unit for prokaryotic and eukaryotic subunits as prokaryotic ribosomes are 70s and eukaryotic ribosomes are 80s so they stop sedimenting at respective sedimentation coffecient in the gradient. Hence can be seperated thereof

gladiator answered 2 years ago

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