Question

In: Biology

i want introduction of LIPASE ASSAY (USING TURBIDIMETRIC METHOD)

i want introduction of LIPASE ASSAY (USING TURBIDIMETRIC METHOD)

Solutions

Expert Solution

Lipases are enzyme that can split fat by breaking the tri-glycerides into fatty acid and glycerol.

Lipase assay is a measure of the enzymatic activity of the enzyme. Here the substrate of a enzyme (like triglycerides, olive oil, triolein) is mixed in a suitable buffer in presence stabilizing agent (like 2-ethoxyethanol) to create an emulsion with a homogenous dispersion of micelles. Within the emulsion the addition of lipase begins the process of hydrolysis of fat also decreases the number of micelle present in the emulsion. Commercial grade purified lipase can be as a measure of standard lipase activity.

Higher wavelength near about 546nm can be used to measure the turbidity. The scattering of light is a measure of turbidity also the activity of the enzyme.At lower wave length the absorbance of light by the emulsion can significantly effect the the outcome of the experiment.hence other wave length can be used depends on the performed experiment.

So the main basis of the assay is the relationship between the change in turbidity and the number of micelle in the emulsion.

purity of substrate and enzyme, pH and homogeneity of the emulsion, temperature and wave length are the major factors can effect the results of lipase assay.


Related Solutions

please i went introduction about method of enzyme assay for report
please i went introduction about method of enzyme assay for report
Could you explain to me how TRAP assay works? I want to detect telomerase activity of...
Could you explain to me how TRAP assay works? I want to detect telomerase activity of breast cancer cell after the cell treated with anti-tumor (curcumin).
i want an introduction and summery about prestressed concrete to write in my assignment
i want an introduction and summery about prestressed concrete to write in my assignment
I want introduction to talk about the overview of the system of washing clothes, it just...
I want introduction to talk about the overview of the system of washing clothes, it just small program not big project yes it is program language but I just want the requirement of this system, contain an introduction and use case
One advantage of using DNS assay of using DNS assay to detect maltose production is the formation of a _______ and ________ compound?
One advantage of using DNS assay of using DNS assay to detect maltose production is the formation of a _______ and ________ compound?
I want a project report about Rate of Return Analysis with references please. - Introduction -...
I want a project report about Rate of Return Analysis with references please. - Introduction - importance of rate of return to stakeholders ​​​​​​​- Benefits of rate of return - analysis of rate of return - examples - application of rate of return - conclusion
I want a 10 page introduction and literature review on “ACHIEVING ECONOMIES OF SCALE AND SCOPE...
I want a 10 page introduction and literature review on “ACHIEVING ECONOMIES OF SCALE AND SCOPE THROUGH STRATEGIC REACTIVATION OF THE RADIOLOGY DEPARTMENT; -THE CASE OF MY HOSPITAL. Background: My hospital has been without a functional radiology department for the past at least 5 years because they could not get a specialist doctor Radiologist to run the department. All clients requiring such radiological services were outsourced to nearby facilities leading to loss of revenue. Management, a few years ago, strategically...
Introduction: Pill bugs In today’s exercise, we will be using the scientific method to study the...
Introduction: Pill bugs In today’s exercise, we will be using the scientific method to study the behavior of the pill bug - Armadilidium vulgare. Pill bugs are also called woodlice and roly-poly bugs. They aren’t actually insects but belong to the subphylum Crustacea which means that they are more closely related to shrimp and lobsters. Pill bugs are found in a variety of habitats including forests, grasslands, and urban areas. They prefer dark, moist areas and are often found under...
Hello, I am using BASH. I need to write a conditional statement using grep. I want...
Hello, I am using BASH. I need to write a conditional statement using grep. I want the computer to echo true if it detects any numerical character in the first line of StrepList.txt. However, the computer tells me that "grep: [0-9]: No such file or directory" if (head -n 1 StrepList.txt | grep -o [0-9] -eq TRUE);then echo "Contains numbers" else echo "No numbers" fi
Write an essay on buyers using the method of division-classification. (500 words) Write an introduction to...
Write an essay on buyers using the method of division-classification. (500 words) Write an introduction to engage the attention of the reader leading to a thesis statement with a hint to the plan of development of your essay in three supporting points, develop each of the supporting points in a paragraph, and write a conclusion.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT