please i went introduction about method of enzyme assay for report

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queen_honey_blossom answered 3 months ago

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A colorimetric LDH assay provides a method to measure the enzyme activity of LDH. NADH (MW...

A colorimetric LDH assay provides a method to measure the enzyme activity of LDH. NADH (MW 633.4 g/mol) absorbs light at 340 nm while NAD+ does not show any absorption at that wavelength. The Beer-Lambert Law relates the absorbance of a solute to its concentration: A = ε*c*L A is absorbance, ε is the molar extinction coefficient, c is concentration, and L is the path length of the solution in the spectrophotometer (usually in cm). In the lab you have...

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