Explain how to: -Make a 1/10 dilution of your original yeast suspension. -Now make 2 fold...

Explain how to:

-Make a 1/10 dilution of your original yeast suspension.

-Now make 2 fold dilutions until you have 6 more standards. You have already counted your original sample so you will be able to calculate the cell density for each standard.

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Expert Solution

Answer:

Procedure to make 1/10 dilution:

Take 100ul (microliter) of the original yeast suspension and add 900ul media.
Total volume obtained = (100 + 900 = 1000ul) yeast suspension and dilution (DF = 1/10)

Procedure to make 2 fold serial dilutions until 6 more standards:

Dilution 1: Add 500ul from 1/10th dilution prepared about and add 500ul media (DF = 1/2)
Dilution 2: Add 500ul from Dilution 1 and add 500ul media (DF = 1/2)
Dilution 3: Add 500ul from Dilution 2 and add 500ul media (DF = 1/2)
Dilution 4: Add 500ul from Dilution 3 and add 500ul media (DF = 1/2)
Dilution 5: Add 500ul from Dilution 4 and add 500ul media (DF = 1/2)
Dilution 6: Add 500ul from Dilution 5 and add 500ul media (DF = 1/2)

Total dilution factor = (1/10)*(1/2)⁶ = 0.001563

Cell densities for each standard can be determined by dividing the original cell density with cumulative dilution factor. For example for dilution 4, cumulative dilution factor = 1/10 * 1/2 * 1/2 * 1/2 * 1/2 = 0.00625

gladiator answered 2 years ago

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