Explain how to: -Make a 1/10 dilution of your original yeast suspension. -Now make 2 fold...

Explain how to:

-Make a 1/10 dilution of your original yeast suspension.

-Now make 2 fold dilutions until you have 6 more standards. You have already counted your original sample so you will be able to calculate the cell density for each standard.

Expert Solution

Answer:

Procedure to make 1/10 dilution:

Take 100ul (microliter) of the original yeast suspension and add 900ul media.
Total volume obtained = (100 + 900 = 1000ul) yeast suspension and dilution (DF = 1/10)

Procedure to make 2 fold serial dilutions until 6 more standards:

Dilution 1: Add 500ul from 1/10th dilution prepared about and add 500ul media (DF = 1/2)
Dilution 2: Add 500ul from Dilution 1 and add 500ul media (DF = 1/2)
Dilution 3: Add 500ul from Dilution 2 and add 500ul media (DF = 1/2)
Dilution 4: Add 500ul from Dilution 3 and add 500ul media (DF = 1/2)
Dilution 5: Add 500ul from Dilution 4 and add 500ul media (DF = 1/2)
Dilution 6: Add 500ul from Dilution 5 and add 500ul media (DF = 1/2)

Total dilution factor = (1/10)*(1/2)⁶ = 0.001563

Cell densities for each standard can be determined by dividing the original cell density with cumulative dilution factor. For example for dilution 4, cumulative dilution factor = 1/10 * 1/2 * 1/2 * 1/2 * 1/2 = 0.00625

gladiator answered 2 years ago

What would be the concentration of a 16 mM solution after 4–fold dilution? To make 100...

What would be the concentration of a 16 mM solution after 4–fold dilution? To make 100 mL of a 5% (w/v) glucose solution, how much glucose would you use? How much NaCl would you add to make an aqueous 10% (w/v) NaCl solution with a final volume of 500 mL? A 1X reaction buffer contains the following concentrations of each compound. Calculate the amount of each compound (in g) that would be needed to make 100 mL of a 5x...

1. If you had a 1 ml liquid culture, how would you make a 1:10 dilution?...

1. If you had a 1 ml liquid culture, how would you make a 1:10 dilution? A 1:50 dilution? A 1:100 dilution? 2. If you make a 1:100 dilution with a final volume of 1 ml, then use 100 ul or 0.1 ml into 900 ul of H2O. What would be your final dilution?

if 0.1 mL of a 10^-2 dilution is spread on a agar plate, what dilution will...

if 0.1 mL of a 10^-2 dilution is spread on a agar plate, what dilution will it be considered when calculating the original number of cells in the sample?

The dilution used for plating into spread plate was 10^-2 What is the dilution factor to...

The dilution used for plating into spread plate was 10^-2 What is the dilution factor to be used in the formula (CFU x Dilution factor)/plating factor

What is the final dilution of a liquid diluted first by 10^-1, then 10^-2, again at...

What is the final dilution of a liquid diluted first by 10^-1, then 10^-2, again at 10^-2, and finally 10^-1?

2. How would you produce a 10-1 dilution of a 3 ml sample using the entire...

2. How would you produce a 10-1 dilution of a 3 ml sample using the entire 3 ml volume? 3. What is the dilution if 96 ml of diluent is added to 4 ml of bacterial suspension? 4. Plating 1.0 ml of a sample diluted by a factor of 10-3 produced 43 colonies. What was the original cell density in the sample? 5. Plating 0.1 ml of a sample diluted by a factor of 10-3 produced 43 colonies. What was...

The first dilution with me 1/100th of the original concentration. In other words, one hundreth part...

The first dilution with me 1/100th of the original concentration. In other words, one hundreth part of the second solution will be dye and 99 parts will be water. Te final volume of the solution will be 10ml. a) what part of it will be dye? b) what part of it will be water? the second dilution will be 1/10th of the first dilution. The final volume will be 10ml. a) therefore, how many ml of water do you place...

You counted 620 colonies on a plate with a dilution of 10-2. How many cells/ml were...

You counted 620 colonies on a plate with a dilution of 10-2. How many cells/ml were in the original sample?

Of the original “undiluted” sample from Question 1(see below), 10L of a 5-to-1 dilution were used...

Of the original “undiluted” sample from Question 1(see below), 10L of a 5-to-1 dilution were used to measure protein concentration. With the use of a standard curve, this is found to be 140g. What is the specific activity of the original sample? Question 1 referenced above: Assayed for LDH activity were 5L of a sample that was diluted 6 to 1. The activity in the reaction vessel, which has a volume of 3 mL, is 0.30 U. What is the...

In 2-3 sentences, describe howto do a serial dilution of a 1x 10-3Msolution to obtain 1...

In 2-3 sentences, describe howto do a serial dilution of a 1x 10-3Msolution to obtain 1 L of a 1x 10-9M solution. (Include necessary volumes and equipment.)

Question