Question

What part of the inverted microscope is different than on a brightfield microscope?

a. The stage and the specimen

b. The light path

c. The coarse adjustment knob

d. The stage

e. The fine adjustment knob

f. All of these

Answer 1

ANS:- f) All of these.

​​​​The inverted microscope is different than on a brightfield microscope in many way :- 1) The specimen & stage 2) light path (position of light source) 3) coarse and fine adjustment knob.

The working principle of the inverted microscope is basically the same as that of an upright light microscope.

Stage :-

​​​​Inverted microscope have a large fixed stage able to hold large vessels like Petri plates. Specimen stage present in between objective and condenser lens. Objective lens bottom of the specimen stage.

Dual Concentric knobs:-

It has dual concentric knobs : fine and coarse adjustment knobs for fine-tuning and focusing the objectives to the specimen.

Light Source and Light Path:-

Light source of inverted microscope in top of the microscope. In the inverted microscope, the condenser is found on top of the stage pointing down to the stage and the objectives located below the stage and pointing upwards. Light path transmitted different from bright field microscope.

light from the condenser magnifying the image, which is then sent to the ocular lens. Light is reflected by the ocular lens through a mirror which clearly shown that path of light like downward then to upward with angle.

Where , Simple light microscopes are sometimes referred to as brightfield microscopes. It means the specimen is dark and contrasted by the surrounding bright viewing field.Generally, the microorganisms do not absorb light but after staining, the organisms get the ability to absorb the light.

Stage:-

Specimen stage in between objective and condenser lens. Objective lens present top of specimen.

To visualize the magnified image of the specimen first, adjust the condenser lens of the light microscope. A condenser lens plays a significant role in transferring the incident light from the illuminator to the specimen. The condenser lens must be near the specimen.

The coarse and fine adjustment knob:-

It is different from inverted microscope.There are two focusing knobs, the fine and the coarse adjustment knobs which are located on the arm. These are used to move either the stage or the nosepiece to focus the image.

Light source and path:-

The optical lens, i.e. condenser lens and the path of light coming from the light source illuminator produce a bright field image with higher contrast and magnification. Light source present bottom of the microscope. light path upwards and very simple which requires a light source like a halogen lamp, condenser lens, objective lens and ocular lens.