Urea was hydrolyzed with an enzyme at a fixed concentration of 50 μM and the following...

Urea was hydrolyzed with an enzyme at a fixed concentration of 50 μM and the following data were obtained. Using Lineweaver-Burk plot determine the kinetic parameters.

Urea(mM)	0.10	0.20	0.30	0.40	0.50	0.60	0.7	0.80	0.90	1.00
Rate(mMs^-1)	6.25	12.5	18.8	25.0	31.2	37.5	43.7	50.0	56.2	62.5

Expert Solution

By Michaelis-menten kinetics we know

where v= rate of reactiom is the maximum reaction rate [S] is the substrate concentration .But mathematically to fit this model into experimental data

We take a reciprocal of this equation on both sides we get Thus if we plot 1/v vs 1/[S] we will get a straight line with slope and y-intercept as as the model resembles y=mx+c straight line equation

We get ,we get .Thank You!

Amanda K answered 1 year ago

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