As a reminder, PCR is the technique used to replicate DNA in
the lab. PCR is based on the way DNA is replicated in cells.
During PCR template DNA plus other ingredients are placed in a
test tube and the solution goes through repeated cycles of heating
and cooling.
The 'ingredients' of a basic PCR reaction are: DNA template,
primer, nucleotides, DNA polymerase, and any salts required for
polymerase function (buffer).
No ligase is required in PCR but it is...