Question

outline a direct interaction between adipocytes and myocytes and what is the outcome from this interaction

Answer 1

Interactions between adipocytes and myocytes were modelled using coculture system composed of primary human adipocytes and human myotubes derived from lean or obese donors.Metabolic analysis of myocytes was performed after coculture with liolytically silent or activated adipocytes and included transcript and metabolic profiling along with assessment of substrate selection and insulin action.Cocultured adipocytes inceased myotube mRNA expression of genes involved in oxidative metabolism .Adipocytes is the basal state sequestered free fatty acids ,thereby foring neighbouring myotubes to rely more heavily on glucose fuel.Under this condition insulin action was enhanced in myotubes from lean but not obese donors.In contrast when exposed lipolytically active adipocytes,cocultured myotubes shifted substrate use in favou of fatty acids ,which ws accompanied by intracellular accmulation of triacylglycerol and even-chain acylcarnitines,decreased glucose oxidation and modest attenuation of insulin signalling.