Question

Describe the design of the DNA sequence of an artificial chromosome in a typical eukaryote for expressing an engineered transmembrane protein in the plasma membrane. In your answer list the 9 sequence features this DNA fragment requires to work, briefly state the function of each, and briefly indicate what would go wrong if that sequence feature was missing.

Answer 1

DNA sequence of artificial chromosome for transmembrane protein gene that is expressed in the plasma membrane requires

Transmembrane protein gene sequence

Promotor sequence

ER signal

Nuclear export signal

Plasma membrane targeting sequence

Stop signal sequence

Selection marker sequnces

Restriction site sequences

Reason

Gene sequence is the protein coding sequence without that gene cannot be expressed in eukrayote tra sgenic system

Promotor is important for the gene transcription t where polymerase binds and determines the location of the gene to expresses. Without promotor gene cannot be transcribed

Nuclear export signal helps the mRNA produced to be taken out of nucleus to be converted into the protein by the ribosome without that mRNA will stay inside the nucleus

Stop signal sequence important for the transcription half at the end of the protein coding sequence and without stop signal the gene will not be terminated properly results in inactive protein

Plasma membrane targeting sequence is important for the transmembrane protein to localise the protein of interest info plasma membrane and if not present it cannot be targeted to plasma membrane

Selectable marker sequences are important in production of transgenic eukaryote to select for the integration of the gene

Restriction site sequences are important for the gene to be cloned in a vector