Question

In: Other

14.7 Batch and continuous biomass production Pseudomonas methylotrophus is used to produce single cell protein from...

14.7 Batch and continuous biomass production Pseudomonas methylotrophus is used to produce single cell protein from methanol in a 1000-m3 pressure-cycle airlift fermenter. The biomass yield from substrate is 0.41 g/g, KS is 0.7 mg/l, and the maximum specific growth rate is 0.44 1/h. The medium contains 4% (w/v) methanol. A substrate conversion of 98% is desirable. The reactor may be operated in either batch or continuous mode. If operated in batch, an inoculum of 0.01% (w/v) is used and the downtime between batches is 20 h. If continuous operations are used at steady state, a downtime of 25 days is expected per year. Neglecting maintenance requirements, compare the annual biomass production achieved using batch and continuous reactors.

Solutions

Expert Solution


Related Solutions

Batch and continuous biomass production Pseudomonas methylotrophus is used to produce single cell protein from methanol...
Batch and continuous biomass production Pseudomonas methylotrophus is used to produce single cell protein from methanol in a 1000-m pressure-cycle airlift fermenter. The biomass yield from substrate is 0.41 gg-, Ks is 0.7 mg 1", and the maximum specific growth rate is 0.44 h. The medium contains 4% (w/v) methanol. A substrate conversion of 98% is desirable. The reactor may be operated in either batch or continuous mode. If operated in batch, an inoculum of 0.01% (w/v) is used and...
Suspension cultures of Panax ginseng plant cells are used to produce ginseng biomass for the health...
Suspension cultures of Panax ginseng plant cells are used to produce ginseng biomass for the health tonic market. The cells are grown in batch culture in a 2500-litre stirred bioreactor operated at 25 C and 1 atm pressure. Over a period of 12 days, 5.5x10^6 litres of air at 25'C and 1 atm are pumped into the reactor; off-gas containing 5.1x10^5 litres of oxygen leaves the vessel over the same period. About 135 kg of water is lost by evaporation...
1.List the basic sequence of cellular events resulting in protein production and export from the cell,...
1.List the basic sequence of cellular events resulting in protein production and export from the cell, beginning at DNA in the nucleus and ending with vesicular exocytosis. Include the following terms and describe what happens at each organelle or cellular structure: ribosomes, mRNA, DNA, rough the endoplasmic reticulum, exocytosis, nuclear pore complex, Golgi complex (cis and trans sides), nucleus, microtubules, vesicle. 2. Be able to label and describe the function of the cellular features of the eukaryotic mitochondria. 3. Be...
Exotoxin A from Pseudomonas aeruginosa has the exact same activity and host cell target as diphtheria...
Exotoxin A from Pseudomonas aeruginosa has the exact same activity and host cell target as diphtheria toxin. Yet, Pseudomonas aeruginosa infection does not result in the same symptoms or clinical manifestation as diphtheria. Can you think of any reason(s) why this might be the case? Rat cells are known to be resistant to diphtheria toxin. Yet, like humans, EF-2 from rats contains diphthamide. Propose a hypothesis to explain why rat cells are resistant to diphtheria. Can you think of a...
A recombinant protein production in a productionscale process typically has a level of host-cell protein (HCP)...
A recombinant protein production in a productionscale process typically has a level of host-cell protein (HCP) by ELISA of 30 ppm. The most recent batch showed a shift in HCP to 80 ppm.What steps can you take to confirm this result?
What is the main method of biodiesel production, from what biomass material, and what is its...
What is the main method of biodiesel production, from what biomass material, and what is its current contribution to US fuel supplies? Please cite your sources.
50 units of protein A are required per cell to establish a wild-type phenotype. A single...
50 units of protein A are required per cell to establish a wild-type phenotype. A single wild-type allele of gene A produces 40 units of enzyme per cell. Which of the following statements is true regarding the wild type allele. Select ALL that apply. They are haplosufficient They are haploinsufficient They are recessive to null alleles They are dominant to hypomorphs making less than 10 units of enzyme per cell. They are recessive They are recessive to hypomorphs making less...
In order to express a recombinant protein that will be used for antibody production, you are...
In order to express a recombinant protein that will be used for antibody production, you are given the choice to use either a genomic library or a cDNA library. Both are good libraries that have been screened to identify genes and proteins. You decide to use the cDNA library. This is because: a. Introns have been removed from the mRNA and the exons spliced together to create an open reading frame in the transcript. b. The cDNA contains the exons...
Describe the factors of production used to produce Apple iphone?
Describe the factors of production used to produce Apple iphone?
Consider a continuous culture of Bacillus subtilis bacteria, which are used to produce the enzyme amylase...
Consider a continuous culture of Bacillus subtilis bacteria, which are used to produce the enzyme amylase during the growth phase. The relevant parameters for growth of this bacteria are μMAX = 0.88 hr-1, YX/S = 0.5 gdw/gr substrate, S0 = 4.0 gr substrate/L, and the Monod constant KS = 0.3 gr/L, while the protein yield during growth phase is YP/X (aka product parameter α) = 0.12 g protein/gdw. The chemostat is operated at a dilution rate that is 60% of...
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT