Question

In: Advanced Math

A recombinant protein production in a productionscale process typically has a level of host-cell protein (HCP)...

A recombinant protein production in a productionscale
process typically has a level of host-cell protein (HCP) by ELISA of 30 ppm. The
most recent batch showed a shift in HCP to 80 ppm.What steps can you take to confirm
this result?

Solutions

Expert Solution


Related Solutions

In order to express a recombinant protein that will be used for antibody production, you are...
In order to express a recombinant protein that will be used for antibody production, you are given the choice to use either a genomic library or a cDNA library. Both are good libraries that have been screened to identify genes and proteins. You decide to use the cDNA library. This is because: a. Introns have been removed from the mRNA and the exons spliced together to create an open reading frame in the transcript. b. The cDNA contains the exons...
Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell growth. The...
Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell growth. The recombinant protein can be considered a product of cell culture even though it is not secreted from the cells; it is synthesized in addition to normal E. coli biomass. Ammonia is used as the nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula of CH1.55O0.31N0.25. The yield of biomass (excluding recombinant protein) from glucose is measured as 0.48 g...
Explain how elements of the lac operon are exploited to regulate recombinant protein production in E....
Explain how elements of the lac operon are exploited to regulate recombinant protein production in E. coli. this question is biochemistry question
separation processes for bioproducts from E. coli . Recombinant protein production from E. coli resulted in...
separation processes for bioproducts from E. coli . Recombinant protein production from E. coli resulted in the first products from biotechnology. (a) List the primary structures and components of E. coli that must be removed from a fermentation broth to purify a heterologous protein product (one that differs from any protein normally found in the organism in question) expressed for pharmaceutical use. (b) Identify a sequence of steps to purify a conjugate heterologous protein (a compound comprised of a protein...
Describe the stages of an HIV infection overtime (viral replication, target cell population, antibody production, host...
Describe the stages of an HIV infection overtime (viral replication, target cell population, antibody production, host immunity).
1) Describe the process and steps involved in the production of a protein from a DNA...
1) Describe the process and steps involved in the production of a protein from a DNA blueprint.
List an individual-level intervention and an educational production process intervention.
List an individual-level intervention and an educational production process intervention.
List an individual-level intervention and an educational production process intervention.
List an individual-level intervention and an educational production process intervention.
What process would you use to disrupt the primary level of organization of a protein? [Select...
What process would you use to disrupt the primary level of organization of a protein? [Select all that apply] A. Hydrolysis B. Denaturation C. Hydrogenation D. Acidification E. Enzymatic digestion (Protease)
1.List the basic sequence of cellular events resulting in protein production and export from the cell,...
1.List the basic sequence of cellular events resulting in protein production and export from the cell, beginning at DNA in the nucleus and ending with vesicular exocytosis. Include the following terms and describe what happens at each organelle or cellular structure: ribosomes, mRNA, DNA, rough the endoplasmic reticulum, exocytosis, nuclear pore complex, Golgi complex (cis and trans sides), nucleus, microtubules, vesicle. 2. Be able to label and describe the function of the cellular features of the eukaryotic mitochondria. 3. Be...
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT