You Receive a 1.5 mL vial of a 12.5 kDa protein that has a concentration of...

You Receive a 1.5 mL vial of a 12.5 kDa protein that has a concentration of 1.45 mM. When you test its OD280 in a 1cm cuvette, it is 8.434.

How much total protein, in mg, do you have?

A) 0.027 B) 27.2 C) 27187 D) 15.8 E) 158137

Expert Solution

given that 1.5 mL of 1.45mM concentration protein received.

Let us convert this into number of millimoles as follows.

1.5 mL of 1.45 mM protein = 1.5 mL x 1.45 millimole / 1000 mL

= 1.5 x 1.45/1000 millimole

= 0.002175 millimole

given that the protein has the molar mass 12.5 kDa

= 12500 Da

= 12500 g / mol

= 12500 mg / mmol

Therefore 0.002175 mmole protein has the mass = 0.002175 mmol x 12500 mg/mmol

= 27.1875 mg

= 27.2 mg

Hence the option (B) is the Answer.

queen_honey_blossom answered 5 months ago

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