You Receive a 1.5 mL vial of a 12.5 kDa protein that has a concentration of...

You Receive a 1.5 mL vial of a 12.5 kDa protein that has a concentration of 1.45 mM. When you test its OD280 in a 1cm cuvette, it is 8.434.

How much total protein, in mg, do you have?

A) 0.027 B) 27.2 C) 27187 D) 15.8 E) 158137

Expert Solution

given that 1.5 mL of 1.45mM concentration protein received.

Let us convert this into number of millimoles as follows.

1.5 mL of 1.45 mM protein = 1.5 mL x 1.45 millimole / 1000 mL

= 1.5 x 1.45/1000 millimole

= 0.002175 millimole

given that the protein has the molar mass 12.5 kDa

= 12500 Da

= 12500 g / mol

= 12500 mg / mmol

Therefore 0.002175 mmole protein has the mass = 0.002175 mmol x 12500 mg/mmol

= 27.1875 mg

= 27.2 mg

Hence the option (B) is the Answer.

queen_honey_blossom answered 10 months ago

A drug for IV infusion is supplied as a 4 mL vial with a concentration of...

A drug for IV infusion is supplied as a 4 mL vial with a concentration of 2.5 mg /mL. the contents of the vial are transferred into an infusion bag to make a final volume of 1000 mL. The infusion is to run for a total of 120 minutes with a flow rate of 30 mL /hour. How much drug will be delivered?

A drug for IV infusion is supplied as a 10 mL vial with a concentration of...

A drug for IV infusion is supplied as a 10 mL vial with a concentration of 10% w/v. 5 mL of this vial is transferred into an infusion bag to make a final volume of 250 mL. The infusion rate is to be 10 mL / min. How long will it take to deliver a dose of 100 mg of the drug?

A patient is ordered 40 mg IV once daily. the concentration of the vial is 25mg/ml....

A patient is ordered 40 mg IV once daily. the concentration of the vial is 25mg/ml. the IV manual states to dilute the medication in a 50 ml bag of NS and infuse over 30 min. The drop factor is 10gtt/ml. At what rate in drops/min would you infuse the medication?

We have a solution of protein that has a concentration of 0.25 mg/ml. a.We need 20...

We have a solution of protein that has a concentration of 0.25 mg/ml. a.We need 20 g of the protein for an experiment. What volume of the protein solution do we need? b.Suppose we want a solution containing 150 g of the protein at a concentration of 0.50 mg/ml. To do this we will first evaporate the liquid from enough of the protein solution to get 150 g. How much solution do we need to start with? How much H2O...

A drug for injection is supplied as a vial has a concentration of 5% w/v. 10...

A drug for injection is supplied as a vial has a concentration of 5% w/v. 10 mL of this vial is injected into an infusion bag to make a final volume of 500 mL. What is the concentration of the drug in the infusion bag?

You have a mixture of the proteins listed below. Protein pI Molecular Weight (kDa) A 3.1...

You have a mixture of the proteins listed below. Protein pI Molecular Weight (kDa) A 3.1 265 B 6.9 93 C 10.3 96 D 7.1 43 E 8.6 189 1) You load the protein mix onto a cation exchange column at pH 5. Next, you apply a "washing" step by passing through buffer at pH 5. Finally, for your elution step, you apply a pH gradient starting from pH 2.0 to pH 13.0. (A gradient buffer system allows you to...

You are given a vial of cells at a density of 6.8 x 10^6 cells/ml. You...

You are given a vial of cells at a density of 6.8 x 10^6 cells/ml. You are asked to seed a 12 well plate, which requires 2 x 10^5 cells per well. Because every well requires a final media volume of 1mL, your professor asks you to dilute your cells with media so that you can directly add 1ml of diluted cells per well in the plate. How you would dilute your cells so that each well receives 2 x...

You have a protein of unknown concentration (23,503 Daltons), and you want to determine its concentration...

You have a protein of unknown concentration (23,503 Daltons), and you want to determine its concentration using the Bradford assay. You prepare a standard curve by making dilutions of a standard protein solution at 10 mg/mL, using the volumes noted below as “μL stock”, and then adding water to a final volume of 1 mL for each of the dilutions (this is also what you did in Lab 1). You add 10 μL of each dilution to 990 μL of...

You have a protein of an unknown concentration but you know that the range is 0.2...

You have a protein of an unknown concentration but you know that the range is 0.2 mg/mL to 2 mg/mL. You know that typically amounts around 10 µg give a good result on an SDS-PAGE gel. You have 9 wells (1 ladder and 9 others). Using 5 wells, how would you design an experiment to look at the effect of amount of protein loaded on the band appearance on the gel? Remember that you are mixing 10 µL of sample...

You are given a sample that has 1.3 mg/ml of purified protein. The molecular weight is...

You are given a sample that has 1.3 mg/ml of purified protein. The molecular weight is 37,000 Daltons. a) the absorbance in a 1cm cuvette is 0.93. calculate the molar extinction coefficient. Be sure to express your answer with correct units. b)also calculate the extinction coefficient for concentration units of mg/ml, i.e. with units of cm-1 (mg/ml)-1 c) The sample is actually contaminated with 0.01 mg/ml DNA, but the protein concentration, 1.3 mg/ml is correct. explain how the contaminating DNA...

Question