You have total RNA extract from plant. How can you make cDNA only from mRNA selectively?

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Expert Solution

1. mRNA is isolated using MACS Technology from total RNA.

2. cDNA is prepared selectively from Reverse Transcription Poly Chain Reaction (RT-PCR).

Magnetic-activated cell sorting (MACS) is a method for separation of various cell populations depending on their surface antigens (CD molecules). In MACS technology, mRNA will be isolated .

Reverse Transcription Poly Chain Reaction : (RT-PCR) :

Reverse transcription Poly Chain Reaction uses mRNA rather than DNA as the starting template.

The enzyme reverse transcriptase uses the mRNA template to produce a complementary single-stranded DNA strand called cDNA in a process known as reverse transcription.
DNA polymerase is used to convert the single-stranded cDNA into double-stranded DNA.

These DNA molecules will be used as templates for a Poly Chain reactions.

Reverse transcribed cDNA synthesized under carefully controlled conditions is a faithful and stable.

gladiator answered 1 year ago

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