Question

In: Chemistry

Prokaryotic DNA Pol III cannot catalyze nick translation. Why? a. It lacks 3'-5' exonuclease activity b....

Prokaryotic DNA Pol III cannot catalyze nick translation. Why? a. It lacks 3'-5' exonuclease activity b. It lacks 5'-3' exonuclease activity c. It lacks 3'-5' endonuclease activity e. It lacks 5'-3' endonuclease activity e. none of the above

Which of the following enzyme activity enables DNA Pol I to edit its mistakes? a. 3'-5' exonuclease activity b. 5'-3' exonuclease activity c. 3'-5' endonuclease activity d. 5'-3' endonuclease activity e. none of the above

How is mRNA translated? a. 5' 3' direction b. 3' 5' direction c. amino-to-carboxyl direction d. carboxyl-to-amino direction e. none of the above

Which of the following processes is not a part of gene expression? a. replication b. transcription c. processing of mRNA d. translation e. none of the above

Solutions

Expert Solution

1) b. It lacks 5'-3' exonuclease activity

Prokaryotic DNA Pol III cannot catalyze nick translation, because it lacks 5'-3' exonuclease activity. Prokaryotic DNA Pol I catalyze nick translation due to 5' -3' exonuclease activity.

Which of the following enzyme activity enables DNA Pol I to edit its mistakes?

2) a. 3'-5' exonuclease activity

In, prokaryotic all three DNA polymerases have the ability to proofread, using 3’-> 5’ exonuclease activity. The 3'-> 5' exonuclease activity of DNA polymerase I proofreads newly synthesized DNA, removing mismatched nucleotides.

3) c. amino-to-carboxyl direction

The sequence of nucleotides in the template

m- RNA chain determines the sequence of amino acids, which are generated in amino acid chain. Addition of an amino acid occurs at the C-terminus of the peptide and thus translation is said to be amino-to-carboxyl directed.

4) a. Replication

Replication is not a part of gene expression. It is a part of cell division. It is a process that happens when DNA needs to be divided in mitosis.


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