Question

Microbiology actually:
Agglutination test

what type of antigen is detected in each reaction (A, B, C, D, F and G)?

In this experiment latex particles are covered with IgG specific to Strep antigen. what is the purpose of this?

Answer 1

Agglutination test – The latex agglutination test is done in order to check if certain antibodies or antigens are present in the body fluid. In this test, there are 6 reagents namely A,B,C,D,F and G provided for finding out the antigens and this test is used majorly for identification of the Lancefield group of streptococci. So, in each of the reagent it is identified if the antigen is haemolytic or non-haemolytic and which variant of streptococci. This helps in finding the Lancefield antigens.

In this latex particles are covered with IgG specific to Strep antigen as the Strep antigens are soluble and this helps in using Lancefield group to classify the streptococci antigens. This is done so that the Pathodx test gives a positive result and in this nitrous acid is used for extracting antigens from the primary plates of culture