Is there a relationship between absorbance and polarity or
wavelength and polarity? For example, chlorophyll is...
Is there a relationship between absorbance and polarity or
wavelength and polarity? For example, chlorophyll is more polar
than carotene and chlorophyll has higher lambda max of 661 than
carotene with 449 lambda max.
Let's say for a chlorophyll solution we found the absorbance,
and use d that with Beer's law to find the concentration. How come
that concentration is different if we find the concentration of the
same chlorophyll solution using the fluorescence?
What is the wavelength of the maximum absorbance of the
nanoparticles? To what colour of visible light does this
correspond? Estimate a size for the gold nanoparticles formed.
The linear relationship between the variables in Beer’s Law does
not hold for absorbance values over 2.0. Briefly explain an
experimental strategy you would use if a protein solution of
unknown concentration gives you an absorbance reading of
>2.0.
The linear relationship between the variables in Beer’s Law does
not hold for absorbance values over 2.0. Briefly explain an
experimental strategy you would use if a protein solution of
unknown concentration gives you an absorbance reading of
>2.0.
Describe the relationship between wavelength and frequency for
electromagnetic radiation. What is the principle difference between
a radio wave, light, and x-rays?
For your indicator, what is the wavelength of maximum absorbance
for the pH < 4.00 solution? What is the wavelength of maximum
absorbance for the pH > 10.00 solution? What colors correspond
to these wavelengths? How do the observed colors of these solutions
relate to the colors at their absorbance maxima? This is the
procedure:
http://www3.chem21labs.com/labfiles/UofC_GL18_Lab.pdf?rf=5204
For your indicator, what is the wavelength of maximum absorbance
for the pH < 4.00 solution? What is the wavelength of maximum
absorbance for the pH > 10.00 solution? What colors correspond
to these wavelengths? How do the observed colors of these solutions
relate to the colors at their absorbance maxima?
This is the procedure:
http://www3.chem21labs.com/labfiles/UofC_GL18_Lab.pdf?rf=5204