Use the Beer-Lambert law to explain how a colorimetric assay
works in the spectrophotometer. In your answer, define the
following terms; chromophore, extinction coefficient, absorbance,
transmission and reagent blank.
Most of the spectrophotometers in the teaching lab emit light
of wavelengths between 400-700nm. However some spectrophotometers
also emit light between 200-400nm. Why is this extended range
useful? Provide a specific example.
How can you use a spectrophotometer to perform a qualitative
analysis of a substance?