19. Incubate DNA, restriction enzyme, and buffer at 37 degree Celcius for 30 minutes, then run it...

19. Incubate DNA, restriction enzyme, and buffer at 37 degree Celcius for 30 minutes, then run it out on agarose gel electrophoresis. The procedure is known as;

	A.	Replication
	B.	Transcription
	C.	Translation
	D.	Restriction Digestion

QUESTION 19

20. Billions of DNA copies in few hours, generate substantial amounts of DNA samples, the procedure is known as;

	A.	Molecular Cloning
	B.	None
	C.	Polymerase chain reaction (PCR)
	D.	Transformation

Expert Solution

gladiator answered 2 years ago

Which enzyme/s are used in recombinant DNA technology? ligase polymerase helicase restriction enzyme

Propose a chemical mechanism for the cleavage of double stranded DNA by a restriction enzyme.

Propose a chemical mechanism for the cleavage of double stranded DNA by a restriction enzyme. At a minimum, how many specific domain types must there be and what role does each play. Explain the role of any cofactors necessary in the reaction.

The enzyme required for transcription is * RNA polymerase Restriction enzymes Splicesome DNA polymerase

The restriction enzyme ApaI recognises and cleaves the sequence GGGCC^C (^ indicates where the DNA is...

The restriction enzyme ApaI recognises and cleaves the sequence GGGCC^C (^ indicates where the DNA is cleaved). You previously determined the size of the product amplified in the PCR. which is the region between the bold section TGGGCTAGGTGTAGGGGTCCTGAGTTCCGGGCTTTGCTACCCAGCTCTTGACTTCTGT TTCCCGATTTTA AATGAGCAGTTTGGACTAAGCCATTTTTAAGGAGAGCGATGGGGAGG GCTTCCCCCTTAGCACAAGGGCAGCCCTGGCCCTGGCTGAAGCCCAACCCCAACCTC CAAGACTGTGAGAGGATGGGGACTCATCCCTGGAGGAGGTGCCCCTCCTGGTATTGAT AAAGAATGCCCTGGGGAGGGGGCATCACAGGCTATTTGAACCAGCCCTGGGACCTTG GCCACCTCAGTGTCACTGGGTAGGGGGAACTCCTGGTCCCTTGGGTATATGGAAGGTA TCAGCAGAAAGCCAGCACTGGCAGGGACTCTTTGGTACAATACCCAGCATGCATGCTG TGCCAGGGGCTGACAAGGGTGCTGTCCTTGGCTTCCCCATTTTGGAGTGGTCACTTG CCTCTACTCCAGCCCCAGAAGTGGAAACTGAGATGATGTGTGGAGGAGAGAGCCAGC GTTCATGTTGGGAATCTTGAGGCTCCTTTCCAGCTCTCAGATTCTGTGATGCTCAAAGG GTGAGCTCTGTGGGCCCAGGACGCATGGTAGATGGAGCTTAGTCTTTCTGGTATCCAG CTGGGAGCCAAGCACAGAACACGCATCAGTGTTTATCAAATGACTGAGGAAATGAATGA ATGAATGTCTCCATCTCAACCCTCAGCCTGGTCCCTCCTTTTTTCCCTGCAGTTGGTAC AGATGGCATTGTCCCAGTCTGTTCCCTTCTCGGCCACAGAGCTTCTCCTGGCCTCTGC CATCTTCTGCCTGGTATTCTGGGTGCTCAAGGGTTTGAGGCCTCGGGTCCCCAAAGGC CTGAAAAGTCCACCAGAGCCATGGGGCTGGCCCTTGCTCGGGCATGTGCTGACCCTG GGGAAGAACCCGCACCTGGCACTGTCAAGGATGAGCCAGCGCTACGGGGACGTCCT GCAGATCCGCATTGGCTCCACGCCCGTGCTGGTGCTGAGCCGCCTGGACACCATCCG GCAGGCCCTG Now, identify the ApaI site within this region. If you digest the PCR product with ApaI, how many fragments of DNA would you...

A Physician orders 40 mL of an IV solution containing ampicillin to run in 30 minutes...

A Physician orders 40 mL of an IV solution containing ampicillin to run in 30 minutes with a microdrop infusion set. This is to be followed by 500 mL of NS to infuse at 50 drops per minute with an infusion set of 20 gtt/mL. What is the flow rate for the ampicillin solution? What is the total infusion time for the two IV solutions?

For the following Grouped Frequency Distribution below : class 7-12 13-18 19-24 25-30 31-36 37-42 43-48...

For the following Grouped Frequency Distribution below : class 7-12 13-18 19-24 25-30 31-36 37-42 43-48 f 5 6 2 4 1 3 7 1) Find ( Mean , Mode , Standard Deviation , Variance ) 2) Sketch : Histogram , Polygon , and an Ogive

For the stress data given below with the nearest error of 1: 27-17-11-24-36-13-29-22-18 23-30-12-46-17-32-48-11-18 18-32-26-24-38-24-15-13-13 18-21-27-20-16-15-37-19-19...

For the stress data given below with the nearest error of 1: 27-17-11-24-36-13-29-22-18 23-30-12-46-17-32-48-11-18 18-32-26-24-38-24-15-13-13 18-21-27-20-16-15-37-19-19 a) Construct a frequency distribution table. b) Construct the three types of statistical graphs. c) Determine the (1) Mean, (2) Median, (3) Mode, (4) Range, Variance, and (6) Standard Deviation.

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