In: Chemistry
1. If the enzyme-catalyzed reaction E + S ↔ ES ↔ E + P takes place near Vmax to the enzyme (E), what can be concluded about the relative concentrations of S and ES?
a) High [S], [ES] is low
b) High [S], [ES] are at their maximum
c) Low [S], [ES] is low
d) Low [S], [ES] is at its maximum
2. Which of the following statements about Michaelis-Menten kinetics is correct?
a) Km = the substrate concentration required for the reaction to reach Vmax
b) Km = dissociation constant of the enzyme-substrate complex
c) Km = is a measure of the substrate's affinity for the enzyme
d) Km = expression of reaction rate
3. What is the Km value if the substrate concentration is 25 mM and the initial reaction rate is half Vmax for an enzyme catalyzed reaction?
a) Km = 50 mM
b) Km = 12.5 mM
c) Km = 625 mM
d) Km = 25 mM