tsi result. A/NR. (acidic slant/ no reaction butt) the teacher say that this may not be...

tsi result. A/NR. (acidic slant/ no reaction butt) the teacher say that this may not be a reliable result. why?

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Expert Solution

TSI test:

TSI test, differentiates fermentation capabilities of carbohydrates sources. It differentiates enteric bacteria, mainly faculative mainly enteric bacteria.

Triple Sugar Iron (TSI) Agar is used.
It is a differential media containing Glucose: Sucrose: Lactose in ratio= 0.1:1:1
Determine both carbohydrate fermentation and hydrogen sulfide H₂S, production in enteric bacteria
Results indicated as:

TSI results:

K= Alkaline

A = Acid

NC = No change

representation	interpretation
K/K or red/red	No sugar fermentation
K/A or red/yellow	Only glucose fermentation
A/A or yellow/yellow	Fermentation of glucose and lactose/sucrose sugars
NC/NC	Less growth, no fermentation or peptone utilization.
K/ NC	No sugar fermentation, superficial utilization of peptone.
A/ NC	Superficial growth with fermentation.

Gas production = bubble/crack

H₂S Production= Black coloration/ deposit

Thus, fermentation only on the slant region may be due to contamination and is not reliable to state result for facultative bacteria or enteric bacteria differentiation.

gladiator answered 10 months ago

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