tsi result. A/NR. (acidic slant/ no reaction butt) the teacher say that this may not be...

tsi result. A/NR. (acidic slant/ no reaction butt) the teacher say that this may not be a reliable result. why?

Expert Solution

TSI test:

TSI test, differentiates fermentation capabilities of carbohydrates sources. It differentiates enteric bacteria, mainly faculative mainly enteric bacteria.

Triple Sugar Iron (TSI) Agar is used.
It is a differential media containing Glucose: Sucrose: Lactose in ratio= 0.1:1:1
Determine both carbohydrate fermentation and hydrogen sulfide H₂S, production in enteric bacteria
Results indicated as:

TSI results:

K= Alkaline

A = Acid

NC = No change

representation	interpretation
K/K or red/red	No sugar fermentation
K/A or red/yellow	Only glucose fermentation
A/A or yellow/yellow	Fermentation of glucose and lactose/sucrose sugars
NC/NC	Less growth, no fermentation or peptone utilization.
K/ NC	No sugar fermentation, superficial utilization of peptone.
A/ NC	Superficial growth with fermentation.

Gas production = bubble/crack

H₂S Production= Black coloration/ deposit

Thus, fermentation only on the slant region may be due to contamination and is not reliable to state result for facultative bacteria or enteric bacteria differentiation.

gladiator answered 2 years ago

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