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In: Biology

Describe what you can see under a microscope for each one. Frog blood smear Reptile blood...

Describe what you can see under a microscope for each one.

Frog blood smear

Reptile blood

Trypanosoma lewisi blood smear rat

iron hemosiderin TB reaction

Human blood smear wright

Expert Solution

1. FROG BLOOD SMEAR:

RBCs of the frog blood has a nucleus unlike the Human Blood, and so it can also divide. So due to the presence of nucleus we can attempt many studies on the DNA using the Frogs’ blood.
RBCs have a more elliptical shape, whereas humans have a more regular and biconcave shaped RBCs.
WBCs and Platelets are lesser in number than the RBCs.

2. REPTILE BLOOD.

RBCs are the largest among the vertebrae, ellipsoidal in shape, present at the centre of the cell. They also have nucleus.
WBCs are present granulocytic or agranulocytic. There are some special type of WBCs in reptiles called as AZUROPHILES. Azurophiles are unique type of WBCs that are found only in reptiles and they’re also called as Primary Granules.
Platelets are also found less in number.
Most commonly used stain is Wright’s Stain.

3. Trypanosoma lewisi blood smear rat:

Typical finding of trypanosomiasis infection by trypanosoma lewisii in rat, in the rat blood smear will be the presence of the adult trypanosoma.
It has a kinetoblast, a nuclues and a flagella.

4. IRON HEMOSIDERIN TB REACTION:

This is also called as Pearl’s Prussian blue reaction
This reaction is used to stain Hemosiderin( the iron storage complex)
It detects the Fe³⁺ Iron in the tissues, no the iron which is bound as hemoglobin.
In patients with TB - Tuberculosis, there is increased staining because of increase Fe³⁺ state of Iron, because this is the state of iron that is required by the TB causing bacteria: Mycobacterium tuberculosis to survive.
So Iron in this state is used uo causing anemia in the long run.

5. HUMAN BLOOD SMEAR WRIGHT:

It is used to differentiate the different blood cells in the human
We can identify the presence of Red blood cells, White blood cells and the platelets
Note the normal values of each.
Note the size and shale of each.

Note: For further clarifications please comment. I tried my best to help you out, hope

I was of use. All the best!

gladiator answered 1 year ago

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