Question

Summarize the methods of performing a phylogenetic analysis--- from obtaining the DNA sequence data to making and editing a phylogenetic tree. How would you calculate intraspecific and interspecific divergences?

Answer 1

The methods of performing a phylogenetic analysis - from obtaining the DNA sequence data to making and editing a phylogenetic tree are-

There are four major steps that are involved-

• The homologous DNA sequence between different species are identified and acquired or any protein sequences which is similar

• These sequences are then aligned.

• A rough tree is predicted on the basis of these sequences

• Then the tree is clearly modified , analysed and then final is made for clear interpretation.

The way by which we can calculate interspecific and intraspecific divergences are-

• We can calculate nucleotide frequency difference to identify sequence homogenization at the monomeric level to exploit the differences in between species.

• The protein sequences can also be studied to calculate the specific divergences so that the individuals can not only separated on the basis of differentiable characters but also at genetic level as well .

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