Question

Draw a typical eukaryotic gene. Be sure to include and label the core promoter, some proximal promoter elements, one enhancer,transcription start site, two introns, poly(A) addition signal, coding region, and untranslated regions. Below the gene draw a full-length primary transcript of the gene with the 5’ and 3’ ends labeled, and below that draw the final mature mRNA with the ends and coding sequence labeled. Be sure it is clear which parts of the gene are represented in the primary transcript and the mRNA.

Answer 1

Eukaryotic gene regulation is slightly complex than prokaryotic gene due to its complexity in DNA ( having histone protein).

In gene there are several sequence of nucleotide present having different function .

Core promoter : It is a short DNA sequence (TATA box) responsible for accurate initiation of transcription.This region have 60 to 120 bp of nucleotide. It contain start site . The main function of promoter is to bind with RNA polymerase and initiates mRNA transcription.

Enhancer :As name suggests it is responsible for enhancing the transcription.It is generally present in upstream,coding region and downstream of a gene.It is cis-acting in nature so always works as positive regulator.

Start site:As we know transcription always occur in 5' to 3' direction ,so start site is always present in 5' direction.

Introns:They are the non coding part of sequence.Always removed by RNA splicing before RNA maturation.

Coding region: This is the main region of gene sequences, that are capable of transcription and translation.In this region Exons are present.

UTR region:Always found in both 5' end and 3' end.5' UTR is called leader sequence and 3' called trailer sequence.

Drawing :

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