Question

3. If you have colonies of two colors and sizes on your LB/amp/ara +pGLO plate, is there any noticeable pattern in the distribution of these colonies? If so, describe what you see.

4. On which of the four plates would you expect to find wild type E. coli growing? What purpose does this plate serve in the design of this experiment?

Answer 1

ANs-3)on one plate comprises only of  LB media all transformed /non transfprmed bacteria grow abundantly in sheet form,

on plate comprises of LB/amp E.coli do not grow because normal wild type bacteria are sensitive to ampicillin

Third type plate  LB/amp/+pGLO. here show white colonies growth of transformed E.coli pGLO occur in presenc eof ampicillin.

fouth type is LB/amp/arabinose+pGLO. here transformed E. coli pGLO growing on LB/amp/arabinose will glow green. Because transformed E. coli have GFP which fluorescence green light using arbinose as a substrate. Arabinose: Induces expression of GFP by binding to the protein AraC. Arabinose creates a differential medium, which means that bothpGLO and non-pGLO cells can grow, but they look different (only the pGLO cells become fluorescent).While Ecoli pGLO does not give green colour when grow on LB plate because of absence of arabinose in media.

ANS2)

normal wild type Ecoli grow on plate 1 that is LB agar plate. this plate act as control in this experiment to check out that wild type grow only on LB agar plate but do not grow on LB/amp plate where only transformed Ecoli can survive so this plate used as control for screening.