Question

In this experiment, you will have to prepare your dosing solutions. Look at the plate layout from your experiment and assume that you need 100 µL of dosing solution per well when dosing 16 wells (this already includes ample surplus) per concentration. Calculate how much MeOH and media is required for each solution.


0% MeOH




1% MeOH

5% MeOH




10% MeOH




20% MeOH




40% MeOH




70% MeOH (double the volume, since you use this dosing solution in your dead wells and your negative control)

Answer 1

According to the information, 16 wells of the plate are required to be filled with a solution containing a requisite percentage of methanol along with culture media. In order to make the required concentrations of solutions, following calculations can be made:

0% Methanol: Add 16*100ul i.e. 1600ul or 1.6ml of culture media.

1% Methanol: Add 1600*1/100 i.e. 16 ul of methanol to 1600-16= 1584 ul of culture media.

5% Methanol: Add 1600*5/100 i.e. 80 ul of methanol to 1520 ul of culture media.

10% Methanol: Add 1600*10/100 i.e. 160 ul of methanol to 1440 ul of culture media.

20% Methanol: Add 1600*20/100 i.e. 320 ul of methanol to 1280 ul of culture media.

40% Methanol: Add 1600*40/100 i.e. 640 ul of methanol to 960 ul of culture media.

70% Methanol: Add 1600*70/100 i.e. 1120 ul of methanol to 480 ul of culture media.

These calculation will made the requisite volumes sufficient to be plated in 16 well, each having 100 ul of volumes.