In: Biology
1. After completing your mutagenesis experiment you set out to determine which gene was responsible. To do so you sequenced the DNA of 100 mutant worms. Listed below is the sequence of a portion of chromosome 1 for 10 of these worms. Circle the nucleotide that is the likely causative mutation for the mutant phenotype.
WILDTYPY SEQUENCE:
5’ AACTCCCTTCTTGGACTACCAGACCTGGGATCCTCTAGCATTCAAGGCT 3’
5’ AACTGCCATCATGGACTACCTGACCTGCGAACCTCTAGCATTCAAGGCT 3’
5’ AACTGCCATCTTCGACTACATGACCAGCCAACCTCTAGCATTCAAGGCT 3’
5’ AACTCCCATCTTGGATTACCTGACCTGCGAACCTCTAGCATTCAAGGCT 3’
5’ AACTCCCATCTTGGACTACCTGACCTGCGAACCTCTAGCATTCAAGGCT 3’
5’ AACTCCCATCTTGGACTACCTGACCTGCGAACCTCTAGCATTCAAGGCT 3’
5’ AATTGCCAACTTGGTCTAGCTGACCTGCAAACTCTAGCATACAAGGCT 3’
5’ AACTGCCATCTTGGACTACCTCACCTGCGATCCTCTAGCAATCAAGGCT 3’
5’ AACTCCCTTCTTGGACTACCAGACCTGCGATCCTCTAGCATTCAAGGCT 3’
5’ AACTGCCATCTTGGACTACCTGACGTGCGATCCTCTAGCATTGAAGGCT 3’
5’ AACTGCCATCTAGGACTACCTGACCTGCCAACCTCTAGCTTTCAAGGCT 3’
Answer : The given seqnece was written in proper format for clustal w alignment & the yellow space marked for wild type sequence number 7 is the mutation. Provided below are the alignment sequence for DNA