Discuss how the cell wall structure of a bacteria determines its gram stain reaction.

Solutions

Expert Solution

Answer-

The basic structute of cell wall in gram positive nacteria is a thick peptidoglycan cell wall ,while in gram negative bacteria the peptidoglycan cell layer is thin and has an additional lipopolysaccharide layer . In gran staining crystal violet dye is used for staining and 95% alvohol to decolorise the bacterial cell . On staining with crystal violet die ,the dye penetrates the cell wall in both gram positive and negative bacteria , later iodine is added to form a large complex of crystal violet. On addition of 95% ethyl alcohol the cell is dehydrated but the crystal violet complex being large cannot escape the cell wall in gram positive bacteria and is retained due to thick cell wall but in gram negative bacteria the lipopolysaccharide layer is degraded along with the thin peptidoglycan layer and the crystal violet die escapes the bacterial cell later on addition of saffranin dye the bacteria turns pink while gram positive appears as purple.

If this helps please give it a positive rating thank you!

gladiator answered 1 year ago

Next > < Previous

Related Solutions

Explain the differences between Gram positive bacteria wall and Gram negative bacteria cell wall.

Compare and contrast gram-positive and gram-negative bacteria, including cell wall and flagella structure. (ESSAY)

The Gram stain differentiates Gram + bacteria from Gram – bacteria because of which of the...

The Gram stain differentiates Gram + bacteria from Gram – bacteria because of which of the following? Group of answer choices presence of an outer membrane in one group and absence in the other presence of teichoic acid in one group and absence in the other presence of ether-linked lipids in one group and absence in the other presence of peptidoglycan in one group and absence in the other difference in thickness of peptidoglycan layer between the two groups

For each type of cell wall in bacteria (gram positive vs. gram negative ) describe the...

For each type of cell wall in bacteria (gram positive vs. gram negative ) describe the unique components present in each and list how each layer of the cell wall is connected together ( what molecules accomplish this)?

What are the differences in cell envelope structure between gram-negative and gram-positive bacteria? How might these...

What are the differences in cell envelope structure between gram-negative and gram-positive bacteria? How might these differences become important if you wish to genetically engineer bacteria to excrete proteins into the extracellular fluid?

4. After performing a Gram stain on a Gram negative bacteria, the specimen shows purple bacteria....

4. After performing a Gram stain on a Gram negative bacteria, the specimen shows purple bacteria. Which of the following best explains why this occurred? A. Crystal violet was not used B. Decolorizer was not used C. The specimen was not heat-fixed D. Safranin was not used 5. Which of the following pieces of evidence could support the endosymbiotic theory if found in some eukaryotes? A. Presence of 80S ribosomes B. The presence of formylated methionine C. Genes that allow...

Describe the structure of the cell envelope of a gram-positive bacteria and compare it with that...

Describe the structure of the cell envelope of a gram-positive bacteria and compare it with that of a gram-negative bacteria. Give detailed answer.

1. Gram postive bacteria differ from gram negative with respect to: A. Cell wall composition B....

1. Gram postive bacteria differ from gram negative with respect to: A. Cell wall composition B. Pepitoglycan content C. The presence of periplasm D. All of the above 2. What advantages does a pour plate have over a streak plate in terms of culture conditions? A. A spread plate in anaerobic and a pour plate is aerobic B. A spread plates is aerobic and a pour plate is anaerobic C. A spread plate places the bacteria equidistance from one another,...

1. how is gram stain done? 2. What types of bacteria are stained differently in the...

1. how is gram stain done? 2. What types of bacteria are stained differently in the Gram stain? 3. What is the key step in the Gram stain that distinguishes between two different cell types? Explain why. The laboratory reported gram-negative bacteria arranged inside the leukocytes of the pus sample. Other tests confirmed the pathogen was Neisseria gonorrhoeae, indicating Tom had the sexually transmitted infection gonorrhea. 4. Describe the shape of the gram-negative bacteria shown in the photo. Dr. Nelson...

Explain the composition of the bacterial cell envelope including plasma membrane (Gram-&+), cell wall (Gram-&+) and...

Explain the composition of the bacterial cell envelope including plasma membrane (Gram-&+), cell wall (Gram-&+) and outer membrane (only Gram-) as well as their individual components. Compare Gram+ and Gram- with regards to differences & similarities.

Subjects