Question

In: Biology

What are the differences in cell envelope structure between gram-negative and gram-positive bacteria? How might these...

What are the differences in cell envelope structure between gram-negative and gram-positive bacteria? How might these differences become important if you wish to genetically engineer bacteria to excrete proteins into the extracellular fluid?

Solutions

Expert Solution

The main difference in cell envelope structure between gram positive and gram negative bacteria is-

  • cell wall of gram positive bacteria is thick ( 20-30 nm) in comparison to gram negative bacteria which is very thin (8-12 nm).
  • gram-positive bacteria contains thick peptidoglycan layer, whereas the peptidoglycan layer is very thin in gram.negative bacteria.
  • cell wall of gram negative bacteria contains an outer wall which is absent in gram positive bacteria.
  • gram negative bacteria contains high amount of lipopolysaccharide ( LPS) in relation to gram.positive bacteria.

when compared for the production of recombinant protein, if gram negative bacteria is used, which contains an outer layer containing lipopolysaccharides that can interfere with the protein secretion into the extracelular medium. Thus resulting in accumulation of secreted protein in periplasm in the form of inclusion bodies. These protein forms are inactive and may remain difficult to recover them back in the active form.

Whereas, in gram-positive bacteria, as they do not have an additional outer layer, the secreted recombinant protein can be released directly into culture medium which makes them more suitable for genetic engeenering.


Related Solutions

Describe the structure of the cell envelope of a gram-positive bacteria and compare it with that...
Describe the structure of the cell envelope of a gram-positive bacteria and compare it with that of a gram-negative bacteria. Give detailed answer.
Explain the differences between Gram positive bacteria wall and Gram negative bacteria cell wall.
Explain the differences between Gram positive bacteria wall and Gram negative bacteria cell wall.
OUTLINE the differences in the envelope structures of Gram-positive and Gram-negative bacteria. You may use diagrams...
OUTLINE the differences in the envelope structures of Gram-positive and Gram-negative bacteria. You may use diagrams or a table. Describe the behavior of chemotaxis in bacteria. Make sure to mention how the flagella contributes and how they can move toward good things and away from bad things. DESCRIBE the Endosymbiotic Theory and OUTLINE the evidence to support
Need a precise rundown of differences between the cell envelope of a Gram-negative bacterial cell and...
Need a precise rundown of differences between the cell envelope of a Gram-negative bacterial cell and a Gram-positive bacterial cell, how the cell membranes and cell walls are different between them and important features.
Compare and contrast gram-positive and gram-negative bacteria, including cell wall and flagella structure. (ESSAY)
Compare and contrast gram-positive and gram-negative bacteria, including cell wall and flagella structure. (ESSAY)
For each type of cell wall in bacteria (gram positive vs. gram negative ) describe the...
For each type of cell wall in bacteria (gram positive vs. gram negative ) describe the unique components present in each and list how each layer of the cell wall is connected together ( what molecules accomplish this)?
1. Describe and/or draw in detail the gram positive and the gram negative bacterial envelope. Then...
1. Describe and/or draw in detail the gram positive and the gram negative bacterial envelope. Then go on to describe all the steps of a gram stain. Conclude by explaining why the gram stain is such an important technique in the microbiology lab.
What are three differences between Gram-positive and Gram-negative cells? (*Not including properties associated with the Gram-stain)....
What are three differences between Gram-positive and Gram-negative cells? (*Not including properties associated with the Gram-stain). Provide an explanation, or description, for each.
1) Create a chart that compares and contrasts the structure of gram-positive and gram-negative cell walls....
1) Create a chart that compares and contrasts the structure of gram-positive and gram-negative cell walls. 2) Gram negative bacteria are considered more harmful than gram positive bacteria.What are the two reasons that contribute to this situation due to the unique characteristic of the outer membrane of Gram-negative bacteria? (Minimum length : 150 words)
Compare and contrast the CELL MEMBRANE of gram-positive and gram-negative cells.Be sure to include function, structure,...
Compare and contrast the CELL MEMBRANE of gram-positive and gram-negative cells.Be sure to include function, structure, and characteristiques in your answer.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT