Question

1. Gram postive bacteria differ from gram negative with respect to:

A. Cell wall composition

B. Pepitoglycan content

C. The presence of periplasm

D. All of the above

2. What advantages does a pour plate have over a streak plate in terms of culture conditions?

A. A spread plate in anaerobic and a pour plate is aerobic

B. A spread plates is aerobic and a pour plate is anaerobic

C. A spread plate places the bacteria equidistance from one another, while a pour plate does not separate cells

D. A spread plate will dry out faster than a pour plate

E. A spread plate exposes organisms to atmosphere oxygen levels while a pour plate also includes a low oxygen environment

3. Pour plates require the agar media to remain molten whilst the culture is added. What will happen if the agar is too hot?

A. The agar media will not set solid

B. The cells in the sample will be killed

C. The cells in the media will all rise to the surface with the heat

4. After performing a serial dilution the cultures may be plated as either spread plates or pour plates?

A. True

B. False

Answer 1

Answer. 1 All of the above, because there is difference cell wall composition, peptidoglycan content and the presence of periplasm.

Answer 2 (E) A spread plate exposes organism to atmosphere oxygen levels while a pour plate also include a low oxygen environment.

Answer 3. (B) The cell in the sample will be killed. Because hot environment abrupt the membrane and kill it.

Answer 4.True. Because after serial dilution we can culture by both methods.