Question

The release of product is the rate-limiting for an enzyme. Therefore:

        A) A burst of product formation is expected         C) A catalytic triad is used to bind substrate
        B) transition state binds weaker than product D) DG°’ for the reaction is positive

Answer 1

Enzymes are molecules of a protein nature that catalyze chemical reactions, provided that they are thermodynamically possible. An enzyme makes a chemical reaction that is energetically possible, but that takes place at a very low speed, is kinetically favorable, that is, it proceeds at a higher speed than without the presence of the enzyme. Like all catalysts, the enzymes work by decreasing the activation energy of a reaction, so that the presence of the enzyme substantially accelerates the reaction rate.
However, the problem suggests that the rate-limiting for the enzyme is the release of the product, this indicates that the action of the enzyme is limited, since it represents the slowest step of the chemical reaction, and is contrary to the function that this one to fulfill, reason why it becomes necessary to help that the enzyme fulfills its real objective to accelerate substantially the rate of reaction (the liberation of the product). Therefore, the use of a catalytic triad is required to bind the substrate and allow the release of the product.

Therefore it is concluded that the answer is the letter (C) A catalytic triad is used to bind substrate