How to determine particle size using UV Visible spectroscopy

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genius_generous answered 6 months ago

Q1. A compound was characterized using UV-visible absorption spectroscopy. The absorbance spectrum of a 1.00 x...

Q1. A compound was characterized using UV-visible absorption spectroscopy. The absorbance spectrum of a 1.00 x 10–4 mol/L solution in a 1.00 cm path length cell peaked at the wavelength of 345 nm to a value of 1.32. (1) What was used as a reference? Specify the cell and its contents. (2) What is the transmittance at that wavelength? (3) What is the molar absorptivity at that wavelength? Q2. Answer the following questions regarding quantitative analysis using UV-visible spectroscopy. Assume...

T/F (TRUE/FALSE) ASAP PLEASEE ANSWER ALL 1.UV spectroscopy is an example of non-radiative decay. Visible spectroscopy...

T/F (TRUE/FALSE) ASAP PLEASEE ANSWER ALL 1.UV spectroscopy is an example of non-radiative decay. Visible spectroscopy relies on radiative decay. This is why we can see colors 2.P-value tells you if a data point is statistically insignificant and can be discarded from a data set. 3.Standard deviation and confidence interval both relate to the scatter around a mean. 4.In fluorescence, emitted photons are released in all directions. Typically they are only detected at a right angle to the irradiating beam...

why does the measurement of fluorescence data require a more intense source of uv/visible spectroscopy? specific....

why does the measurement of fluorescence data require a more intense source of uv/visible spectroscopy? specific. b) what is the typical source for spectofluorometer? c) for the photomuiltiplier transducer what is the mode off operation used signal to noise?

In what form (Fe3+ or FeSCN-) could iron be determined by visible spectroscopy?

Particle in a box is a model that is often used to look at the spectroscopy...

Particle in a box is a model that is often used to look at the spectroscopy of pi electrons in conjugated organic molecules. carotene, a precursor of retinal, a visual pigment found in the retina of the eye, has the formula given below. -carotene contains 11 conjugated double bonds which contribute 22 electrons that are delocalized along the length of the molecular chain. Each level of particle in a box has room for 2 electrons. a. The highest filled energy...

An unknown solution containing a mixture of two compounds is being analyzed by UV/visible spectrometry in...

An unknown solution containing a mixture of two compounds is being analyzed by UV/visible spectrometry in order to determine the amounts of compounds A and B in the sample. Both compounds absorb to some extent at 475 and 630 nm. The calibration curve data obtained during the experiment were analyzed using linear regression analysis. The linear equations for each compound at the various wavelengths are provided in the table below. Results of Linear Regression Analysis Pathlength (cm) 475 nm 630...

What does the “vertical transition” in electronic spectroscopy (UV-Vis) mean and what is the rationale for...

What does the “vertical transition” in electronic spectroscopy (UV-Vis) mean and what is the rationale for this approximation? Please explain and show all work.

Alkanes such as n-hexane are used as solvents in UV spectroscopy. N-hexane absorbs at the wavelength...

Alkanes such as n-hexane are used as solvents in UV spectroscopy. N-hexane absorbs at the wavelength of 1.38x10^-7m (or 138nm) which is within the UV spectral range (300nm-10nm). Why is n-hexane a good choice as a solvent for UV/vis spectroscopy?

There are several distinct differences between IR and UV-Vis spectroscopy. Discuss the following as they relate...

There are several distinct differences between IR and UV-Vis spectroscopy. Discuss the following as they relate to: (a) The types of interactions responsible for the signals generated (b) Source of radiant energy (c) Signal detection (d) Sample cells (e) Sample preparation

Q/ Silicon photodiodes, CCDs, and PMTs are used as detectors (signal transducers) in UV Visible spectrometers....

Q/ Silicon photodiodes, CCDs, and PMTs are used as detectors (signal transducers) in UV Visible spectrometers. Describe the principles of operation of each and their advantages and limitations.

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