You are given 5mL of 3mg/mL protein solution. You are asked to prepare samples for SDS-PAGE...

You are given 5mL of 3mg/mL protein solution. You are asked to prepare samples for SDS-PAGE containing 250ng, 500ng, 750ng, and 1000ng. Your pipettes are calibrated to 2uL at the lower limit. Your total volume is 20uL, with 5uL being your loading buffer. How would you prepare these samples?

Expert Solution

queen_honey_blossom answered 9 months ago

You are given 5mL of 3mg/mL protein solution. You are asked to prepare samples for SDS-PAGE...

You are given 5mL of 3mg/mL protein solution. You are asked to prepare samples for SDS-PAGE containing 250ng, 500ng, 750ng, and 1000ng. Your pipettes are calibrated to 2uL at the lower limit. Your total volume is 20uL, with 5uL being your loading buffer. How would you prepare these samples?

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(8) You have to prepare 250 mL of a 10% (v/v) of SDS solution, which is a detergent commonly used to solubilize proteins. (a) How many mL of SDS will you pipet? (b) How much water will you add?

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This is a question about the SDS - PAGE method, one of the protein analysis methods....

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This is a question about the SDS - PAGE method, one of the protein analysis methods....

This is a question about the SDS - PAGE method, one of the protein analysis methods. 1. I would like to know the functions of resolving gel and stacking gel in SDS - PAGE analysis. 2. I would like to know the various reagents used in manufacturing resolving gel and stacking gel in SDS - PAGE analysis and their role. ( Please tell us in detail the role of each reagent. ) We would appreciate your detailed explanation of each...

Compare and contrast protein (SDS-PAGE) and DNA (agarose) gel electrophoresis.

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Reagents, Supplies and Equipment Laemmli sample buffer containing Dithiothreitol (DTT) at 10 mg/mL 10X tris-glycine-SDS (TGS) electrophoresis buffer Bio-Safe Coomassie stain for proteins Actin and myosin standard Precision Plus Protein Kaleidoscope pre-stained standard Mini-PROTEAN pre-cast polyacrylamide gels Mini-PROTEAN Tetra gel electrophoresis system Microcentrifuge tubes and microcentrifuge tube rack Heat block at 95oC Pipets and pipet tips Procedure Part A – Protein Extraction Using a spatula, put a small amount of fish or meat into a microtube. Weight the tube to...

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You are given 5mL of 3mg/mL protein solution. You are asked to prepare samples for SDS-PAGE...

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You are given 5mL of 3mg/mL protein solution. You are asked to prepare samples for SDS-PAGE...

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