You are given 5mL of 3mg/mL protein solution. You are asked to prepare samples for SDS-PAGE...

You are given 5mL of 3mg/mL protein solution. You are asked to prepare samples for SDS-PAGE containing 250ng, 500ng, 750ng, and 1000ng. Your pipettes are calibrated to 2uL at the lower limit. Your total volume is 20uL, with 5uL being your loading buffer. How would you prepare these samples?

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queen_honey_blossom answered 2 years ago

You are given 5mL of 3mg/mL protein solution. You are asked to prepare samples for SDS-PAGE...

You are given 5mL of 3mg/mL protein solution. You are asked to prepare samples for SDS-PAGE containing 250ng, 500ng, 750ng, and 1000ng. Your pipettes are calibrated to 2uL at the lower limit. Your total volume is 20uL, with 5uL being your loading buffer. How would you prepare these samples?

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This is a question about the SDS - PAGE method, one of the protein analysis methods....

This is a question about the SDS - PAGE method, one of the protein analysis methods. 1. I would like to know the functions of resolving gel and stacking gel in SDS - PAGE analysis. 2. I would like to know the various reagents used in manufacturing resolving gel and stacking gel in SDS - PAGE analysis and their role. ( Please tell us in detail the role of each reagent. ) We would appreciate your detailed explanation of each...

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You are given 5mL of 3mg/mL protein solution. You are asked to prepare samples for SDS-PAGE...

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