What compound will you need to use to visualize the amino acids on thin layer chromatogram?...

What compound will you need to use to visualize the amino acids on thin layer chromatogram?

A student seperates a mixture of octane and 1-octanol using thin layer chromotography. Which compound will move faster with tolunce. Give reason.

You are a chemist working in a lab who is analyzing polar samples with thin layer chromatography. What type of solvent do you think would be ideal for your tests? Why?

Expert Solution

queen_honey_blossom answered 2 years ago

Briefly describe the effect of the following procedural errors on a thin-layer chromatogram. Applying too large...

Briefly describe the effect of the following procedural errors on a thin-layer chromatogram.a) Applying TOO LARGE a sample when spotting the plate.b) Placing the spotted plate into a developing chamber containing an eluent level ABOVE the level of the origin.c) Allowing the plate to remain in the developing chamber until the eluent front reaches the TOP of the plate.d) Removing the plate from the developing chamber when the eluent front has move only HALF-WAY up the plate.e) Developing the plate in an UNCOVERED chamber.

During the metabolism of amino acids, amino groups are often switched from one organic compound to...

During the metabolism of amino acids, amino groups are often switched from one organic compound to another through a process called transamination. The following are two examples of transamination reactions with their corresponding free energy changes: glutamate + pyruvate ---> α-ketoglutarate + alanine ∆Go' = - 1.00 kJ/mol glutamate + oxaloacetate ---> α-ketoglutarate + aspartate ∆Go' = - 4.81 kJ/mol Answer the following questions about transamination. a. Using the two equations given above, write an equation for the net formation...

What is an amino acid? How many amino acids are there?

1)What are amino acids? Amino group, carboxyl end, what is the side chain of an amino...

1)What are amino acids? Amino group, carboxyl end, what is the side chain of an amino acid? 2)What are essential and non-essential amino acid. 3)Which are the essential amino acids? 4)Classify the amino acids based on polarity of the R group:

5. How could you take a protein with a known sequence of amino acids and use...

5. How could you take a protein with a known sequence of amino acids and use it to make an artificial gene?

Experiment 7: Thin Layer Chromatography What is the name of the technique you will be using...

Experiment 7: Thin Layer Chromatography What is the name of the technique you will be using in experiment No.7 lab? Thin Layer Chromatography Mass Spectrometry Thick Liquid Calorimetry Liquid Chamber Chromatography TLC plates separate molecules on the basis of: Polarity Color Size Bond orientation The mixture is dissolved in a fluid called the ___________ phase, which carries it through a structure holding another material called the __________ phase. The polarity of a bond between two elements can be best determined...

Why is it better to use a volatile solvent for the mobile phase of Thin Layer...

Why is it better to use a volatile solvent for the mobile phase of Thin Layer Chromatography

1.How do D -amino acids differ from L-amino acids? What biological roles are played by peptides...

1.How do D -amino acids differ from L-amino acids? What biological roles are played by peptides that contain D -amino acids? 2.Which amino acid is technically not an amino acid? Which amino acid contains no chiral carbon atoms? 3.For each of the following, name an amino acid in which the R group contains it: a hydroxyl group, a sulfur atom, a second chiral carbon atom, an amino group, an amide group, an acid group, an aromatic ring, and a branched...

1. What is the turnover number? How would you calculate it? 2. Which amino acids are...

1. What is the turnover number? How would you calculate it? 2. Which amino acids are small and hydrophobic? Large and hydrophobic? What type of binding site (meaning what amino acids would it contain) would bin a negatively charged amino acid? A positively charged one? A small hydrophobic one? A large hydrophobic one?

Amino acids are classified as essential or nonessential. What does this mean and is the classification...

Amino acids are classified as essential or nonessential. What does this mean and is the classification the same for all organisms?

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