Question

1). You have a patient with a lung infection caused by Mycoplasma pneumoniae. What antibiotic would not work against this organism?

A.Penicillin

B.Tetracycline

C.Streptomycin

D.Erythromycin

E.Ciprofloxacin

2.Arrange the following steps of a cloning experiment in the correct order

1. identify gene of interest

2.transfer recombinant plasmid in a microbe of choice using artificial transformation

3. treat plasmid and inset with restriction endonuclease

4. obtain large quantities of gene of interest using PCR

3.Match the scenarios with the appropriate treatment/agent.

-.minor cuts and breaks in the skin

-.lab media and glassware     

-.antibiotic solutions

-.preparing an injection site

-.preservation of spices and produce

A. autoclava

B.hot air oven

C. pasteurization

D. degerming

E.antiseptic

F. chlorine bleach

G gamma radiation

H.membrane filtration

Answer 1

Answer 1:correct option is (A).

Penicillin would not work against Mycoplasma pneumoniae because  It lacks cell wall around its cell membrane. Due to absence of cell wall, it is resistant to penicillin antibiotics. Penicillin antibiotic inhibits the growth of bacteria by attacking the cell wall. Hence, Mycoplasma pneumoniae is not inhibited by penicillin because it does not have cell wall.

Answer 2:

1: identify gene of interest

2:treat plasmid and inset with restriction endonuclease

3: transfer recombinant plasmid in a microbe of choice using artificial transformation

4:obtain large quantities of gene of interest using PCR

Answer 3:

minor cuts and breaks in the skin--------->antiseptic

lab media and glassware-----------> autoclave

antibiotic solutions------>membrane filtration

preparing an injection site ------> degerming

preservation of spices and produce------> gamma radiation