True or false? Substrate-level phosphorylation is the phosphorylation of AMP by ATP.

True or false?

Substrate-level phosphorylation is the phosphorylation of AMP by ATP.

Expert Solution

Substrate level phosphorylation is the formation of ATP from ADP. In this reaction, a high energy phosphate group is transferred from the phosphorylated intermediate to ADP, resulting in formation of ATP. It is different from oxidative phosphorylation, where ADP binds to inorganic phosphate to form ATP by ATP synthase. Substrate level phosphorylation is seen in TCA cycle, where succinyl CoA is converted to succinate. This reaction is coupled to substrate level phosphorylation of GDP to GTP.

When AMP reacts with ATP, there is transfer of phosphate group to AMP by ATP to form 2 ADP. This reaction is catalyzed by adenylate kinase enzyme. There is no formation of ATP and hence it is not substrate level phosphorylation.

When 2 ADP react to form ATP and AMP, then it is a special kind of substrate levels phosphorylation to generate ATP and AMP.

ATP+ AMP-> ATP is not substrate level phosphorylation.

Right choice: False.

gladiator answered 2 years ago

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