In: Chemistry
Serum taken from a patient being treated with lithium for manic-depressive illness was analyzed for lithium concentration. A reading of 284 units was obtained for the intensity of the 671 nm red emission line. Then 1.00 mL of a 10.5 mM Lithium standard was added to 9.00 mL of serum, and this spiked serum gave an intensity reading of 7.60 × 102 units. What is the original concentration of Li in the serum?
Our first value of units is of the initial concentration.
I(x) = 284; 284 = k[Li+] ----- equation (1), where k is a
proportionality constant.
The next equation is going to represent the initial concentration
of the lithium ion plus the addition of the standard.
I(x+s) = 7.60 x 102; 760 = k*{ [Li+ standard] * (V
standard/ V total) } +k*{ [Li+ serum] * (V initial/ V total)
}
Since we have known that 284 = k*[Li+ serum], we can plug that
value into our equation as---
760 = k*{ [10.5] * (1 mL/ 10 mL) } + 284 * (9 mL/ 10 mL)
=> 760 =k*(1.05) + 255.6
=>k*(1.05) = 504.4
=> k =480.4
Now, putting the value of k in equation(1) we will get the original concentration of Li in the serum as---
equation (1) => 284 =k[Li+]
=> [Li+] = 284/480.4
=> [Li+] = 0.59 mM
{* note that intensity reading is taken as 7.60 × 102 units instead of 7.60 × 102 units}