Question

Chromatograpy Terms

1. Hyphenated systmes

2. leading/ breading

3. Tailing

4. Partion Ratio

5. peak width

6.Retntion Time

7. Adjusted Retention Time

8. Relative Retention Time

9. Retention Factor/Capacity Factor

10. Peak Resolution

Answer 1

1) Hyphenated technique is combination or coupling of two different analytical techniques with the help of proper interface. Hyphenated techniques combine chromatographic and spectral methods to exploit the advantages of both. Chromatography produces pure or nearly pure fractions of chemical components in a mixture. Spectroscopy produces selective information for identification using standards or library spectra.

5) The peak width or the number of theoretical plates in the column (N) is a measure of peak broadening.

6) Retention time is the time a solute takes to move from the point of injection to the detector.

7) Adujusted retention time is the time the analyte spends in the stationary phase. Adjusted retention time is equal to retention time minus elution time of an unretained peak.

8) Relative retention time (RRT) is the ratio of the retention time of analyte peak relative to that of another used as a reference obtained under identical conditions.

9) Rretention factor is the ratio of the distance from the center of the spot for a given mixture component to the distance traveled by the mobile phase.

10) Peak resolution is the separation between two chromatographic bands. It is a measure of the separation of two peaks taking into account both the difference in elution time and the peak widths.