In: Chemistry
How do the partition chromatography model and the adsorption chromatography model differ in their explanations of how separations occur in column chromatography?
Partition Chromatography |
Adsorption chromatography |
|
Separation principal |
In this chromatography compund is seperated based on their partition coffecient between two liquid phase. |
In this chromatography separation is based of different adsorption affinity of compound with stationary phase |
Distribution of compound |
Here polar compound try to go polar liquid phase and non polar compound vice versa |
Here polar compound adsorb more in polar stationary phase and nonpolar compound vice versa |
Stationary phase |
Here stationary phase generally liquid- liquid but Liquid- gas may also be observed |
Here stationary phase is must be solid |
Mobile phase |
Here mobile phase is liquid |
Here also liquid but with changing the solvent polarity different component of mixture elute |
Analytical technic |
high performance liquid chromatography (HPLC), Paper chromatography, gas chromatography (GC) work in this principle |
column chromatography, Thin layer chromatography etc. adsorption chromatography is used. |