Question

In: Chemistry

How do the partition chromatography model and the adsorption chromatography model differ in their explanations of...

How do the partition chromatography model and the adsorption chromatography model differ in their explanations of how separations occur in column chromatography?

Solutions

Expert Solution

Partition Chromatography

Adsorption chromatography

Separation principal

In this chromatography compund is seperated based on their partition coffecient between two liquid phase.

In this chromatography separation is based of different adsorption affinity of compound with stationary phase

Distribution of compound

Here polar compound try to go polar liquid phase and non polar compound vice versa

Here polar compound adsorb more in polar stationary phase and nonpolar compound vice versa

Stationary phase

Here stationary phase generally liquid- liquid but

Liquid- gas may also be observed

Here stationary phase is must be solid

Mobile phase

Here mobile phase is liquid

Here also liquid but with changing the solvent polarity different component of mixture elute

Analytical technic

high performance liquid chromatography (HPLC), Paper chromatography, gas chromatography (GC) work in this principle

column chromatography, Thin layer chromatography etc. adsorption chromatography is used.


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