Question

IL1RL1 is a gene which encodes a functional protein. The NCBI Accession can be found here. https://www.ncbi.nlm.nih.gov/nuccore/NM_016232.5 If Alkylation damage were to occur at base 215 of this sequence, what would be the resulting change, and would that change the function of the protein and to what degree?

Answer 1

Homo sapiens interleukin 1 receptor like 1 (IL1RL1), transcript variant 1, mRNA

human 1,997 bp mRNA sequence NM_016232.5.

it is a transcript sequence which will translate into protein . the protein means sequenced chain of amino acids. The sequence of aminoacids are totally dependent on the sequence of bases on m RNA. Any damage to any base may alter the amino acid sequence and  would that change the structure and function of the protein .

If Alkylation damage were to occur at base 215 of this sequence:-

181 accaatactt gctcttgatt gataaacaga atggggtttt ggatcttagc aattctcaca 240

201 gataaacaga 210 211 atggggtttt 220

gggg :- sequence count 213 to 216 .

This means the 215 base is G. the alkylation damage of G :- Alkylation of guanine at the O6 atom is a highly mutagenic DNA lesion because it alters the coding specificity of the base causing G:C to A:T transversion mutations.

• yes its mutagenic change in structure of protein a function of protein