Copy three sequences and perform a multiple sequence alignment on them. In your alignment, include B73...

Copy three sequences and perform a multiple sequence alignment on them. In your alignment, include B73 and one strain containing the insertion and one that does not.

>B73

CGCAACACATGTTAGCACAAGAGTTGCAGGAACACTGTAAGTGAGCGTCTGCTTTT ATATTTTTTCGATTTCTTCTCTTATTGTTTCTCGAGCGCCTAGAGAAGCTCGTTCTTG TACCATCCTTTTCATTTTAGTTGGATGATACTCCCTCCGTCTCATGATATAAGGCGTA ACCACTTTTTATTCTTGTCCCGCAATATAAGGCGTGCTCTCTCTATGCATACGTATAT CGATGCAGTGGTATAGAGACAATTAAATGTATTTCTTGGTCTTTGAACCAGAGGTGG TTACGCCTTATATACTGGGACGGAGGGAGTACTTGTTAAATTTTAAGAAATGTCCAG AGAGTTCAGCAAAGCAATAACATTTGTACTTACCTGTATATGATATATACATGTGGC CTATGAATACTTTTTTCAGCTTTGCTTACTCCGTTTTAATTGGCAGGGGTTATTTGGC TCCTGAGTATGCTATTCGAGGACAGGTGACACGCAAATCAGATGTGTACAGCTTTG GCGTGCTGCTTCTGGAAATTGTTTGCGGGAGATCAAACAGCGATACAAGATTAGCA TACGGAGATCAGATACTCCTCGAAAA

Sequence with insertion

>Tzi25

CGCAACACATGTTAGCACAAGAGTTGCAGGAACACTGTAAGTGAGCGTCTGCTTTT ATATTTTTTCGATTTCTTCTCTTATTGTTTCTCGAGCGCCTAGAGAAGCTCGTTCTTG TACCATCCTTTTCATTTTAGTTGGATGATACTCCCTCCGTCTCATGATATAAGGCGTA ACCACTTTTTATTCTTGTCCCGCAATATAAGGCGTGCTCTCTCTATGCATACGTATAT CGATGCAGTGGTATAGAGACAATTAAATGTATTTCTTGGTCTTTGAACCAGAGGTGG TTACGCCTTATATACTGGGACGGAGGGAGTACTTGTTAAATTTTAAGAAATGTCCAG AGAGTTCAGCAAAGCAATAACATTTGTACTTACCTGTATATGATATATACATGTGGC CTATGAATACTTTTTTCAGCTTTGCTTACTCCGTTTTAATTGGCAGGGGTTATTTGGC TCCTGAGTATGCTATTCGAGGACAGGTGACACGCAAATCAGATGTGTACAGCTTTG GCGTGCTGCTTCTGGAAATTGTTTGCGGGAGATCAAACAGCGATACAAGATTAGCA TACGGAGATCAGATACTCCTCGAAAAGGTCAAAATGACGCT

Sequence without insertion:

>A679

CGCAACACATGTTAGCACAAGAGTTGCAGGAACACTGTAAGTGAGCGTCTGCTTTT ATATTTTTCGATTTCTTCTCTTATTTTTTTCTCGAGTGCCTAGAGAAGCTCGTTCTTGT ACCATCCTTTTCATTTTAGTTGGATGATACTTGTTAAATTTAAGAAATGTCCAGAGAG TTCCGCAAAGCAATAACATCTGTACTTACCTGTATATGATATATACATGTGGCCTAT GAATACTTTTTTGAGCTTTGCTTACTCCGTTTTAATTGGCAGGGGTTATTTGGCTCCT GAGTATGCTATTCGAGGACAGGTGACACGCAAATCAGATGTGTACAGCTTTGGCGT GCTGCTTCTGGAAATTGTTTGCGGGAGATCAAACAGCGATACAAGATTAGCATACG GAGATCAGATACTCCTCGAAAAGGTCAGATGACGCT

What does this insertion represent (i.e. what type of polymorphism is this and explain

Expert Solution

Question

The result of multiple sequence alignment of three sequences is following :

Figure: MSA result

The insertion represent signify a mutation in which extra nucleotide is added to the nucleotide or protein sequence. Similarly, deletion represent a mutation where one or more nucleotide base get deleted from the sequence.

The mutation is the phenomeon that change the nucleotide or protein sequence. The mutation could lead to deletion, insertion and substitutions. In this question, Insertion and deletion are present that show "indel polymorphism".

gladiator answered 2 years ago

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