A. Once your chromatography paper was in the chamber and the seperation was underway l, why...

A. Once your chromatography paper was in the chamber and the seperation was underway l, why was it essential that you patiently wait for the solvent front to rise nearly to the top of the paper, rather than just halfway?

B. Why was it important to mark the position of the solvent front immediately after removing the paper from the chromatography chamber?

Expert Solution

A.

1.Some times product spot and starting material spot may be close to each other therefore we want to wait for the solvent front to rise nearly to the top of the paper for better sepration.

2. more polar compound will move slowly therefore it is necessary to wait for the solvent front to rise nearly to the top of the paper.

B.

to calculate the retention factor it is necessary to mark the solvent front immediatley after removing the paper from the chromatography chamber.

once the solvent is dried without marking it is difficult to calculate the retention factor

retention factor = distance travel by the compound / distance travelled by the solvent front

queen_honey_blossom answered 1 year ago

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