A. Once your chromatography paper was in the chamber and the seperation was underway l, why...

A. Once your chromatography paper was in the chamber and the seperation was underway l, why was it essential that you patiently wait for the solvent front to rise nearly to the top of the paper, rather than just halfway?

B. Why was it important to mark the position of the solvent front immediately after removing the paper from the chromatography chamber?

Expert Solution

A.

1.Some times product spot and starting material spot may be close to each other therefore we want to wait for the solvent front to rise nearly to the top of the paper for better sepration.

2. more polar compound will move slowly therefore it is necessary to wait for the solvent front to rise nearly to the top of the paper.

B.

to calculate the retention factor it is necessary to mark the solvent front immediatley after removing the paper from the chromatography chamber.

once the solvent is dried without marking it is difficult to calculate the retention factor

retention factor = distance travel by the compound / distance travelled by the solvent front

queen_honey_blossom answered 2 years ago

why is there repulsion at large nucleon seperation?

Compare and contrast Column (solid-liquid) chromatography, Paper Chromatography, and thin layer chromatography in respect to the...

Compare and contrast Column (solid-liquid) chromatography, Paper Chromatography, and thin layer chromatography in respect to the size of the sample that can be separated, the ease of use, and the versatility of the method.

Give two advantages of thin layer chromatography over paper chromatography.

Paper chromatography lab experiment. (color dye) in the solvent system that you use for analyzing your...

Paper chromatography lab experiment. (color dye) in the solvent system that you use for analyzing your unknown a)name the important intermolecular forces acting between the dye and the solvent and b)the dye and stationary phase for (i) the dye with the largest Rf value (ii) the dye with the smallest Rf value. comment on the relative important of the types of the forces that result in observe Rf value. c) what statical test could you apply to give some quatitation...

How pigment separation using paper chromatography works?

In a fractional distillation seperation method, why is it important to insulate the column? What would...

In a fractional distillation seperation method, why is it important to insulate the column? What would be the consequence of not insulating the column during fractional distillation?

why do we raise the pH before the second centrigugering in protein seperation

) Using paper chromatography, the RF value for xanthophyll was 0.3 and the value for chlorophyll...

) Using paper chromatography, the RF value for xanthophyll was 0.3 and the value for chlorophyll was 0.7. What is true for these two pigments? A) Chlorophyll traveled farther up the paper than xanthophyll. B) Xanthophyll is more soluble than chlorophyll a C) Chlorophyll is a larger molecule than xanthophyll. D) Chlorophyll is more polar than xanthophyll.

When doing Paper Chromatography, 1.What would you expect to see if the paper had been touched...

When doing Paper Chromatography, 1.What would you expect to see if the paper had been touched by fingers after developing with solvent, but before staining with ninhydrin? 2.Why does the filter paper need to be stapled so that the edges do not touch each other? What would occur if it were done incorrectly?

How is thin layer chromatography technique of separation similar to a paper chromatographic separation?

Question