1.)Where is the accepted cut off/threshold for the E-value (hint: it is a number).

2.) What is a read and how is it different from a sequence?

3.)How is CDS different from the nucleotide sequence in GenBank?

4.)How many potential reading frames could result from a double stranded DNA?

Expert Solution

1)1e-2 to 1e-30

2)sequence is a generic name describing the order of biological letters (DNA/RNA or amino acids). Both contigs and reads are DNA/RNA or aa sequences. reads are just a shorthand for sequenced reads.

3)The identification of coding sequences (CDS) is an important step in the functional annotation of genes. ... CDS is a sequence of nucleotides that corresponds with the sequence of amino acids in a protein. A typical CDS starts with ATG and ends with a stop codon. CDS can be a subset of an open reading frame (ORF).

4)The genetic code reads DNA sequences in groups of three base pairs, which means that a double-stranded DNA molecule can read in any of six possible reading frames--three in the forward direction and three in the reverse. A long open reading frame is likely part of a gene.

gladiator answered 2 years ago

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