100 mL of a 100 mM Tris at pH 7.8 is diluted to 1L. What is...

100 mL of a 100 mM Tris at pH 7.8 is diluted to 1L. What is the resulting pH?

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Expert Solution

initial volume = 100 ml

initial concentration of Tris = n/v = 100/100 = 1 M

dilution formula, M1V1 = M2V2

(1*0.1) = M2*1

M2 = final concentration of TRIS solution = 0.1 M

after dilution ,

pH of tris = 1/2(pka - logC)

pka of tris = 8.07
= 1/2(8.07-log0.1)

= 4.535

queen_honey_blossom answered 1 year ago

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