What are the steps in detail that you need for the industrial production of an enzyme...

What are the steps in detail that you need for the industrial production of an enzyme that is a membrane bound protein, of which molecular weight is 90 KD and surface charge is positive? Add hand drawn picture as many as possible and confirm the amount of enzyme by ELISA and Western blot.

Expert Solution

Biotechnological process of enzyme production:

1. Screening – Choosing an appropriate micro organism for the desired enzyme.

2. Modification – Possible application of genetic engineering to improve the microbial strain

3. Laboratory scale pilot – To determine the optimum conditions for growth of micro-organisms.

4. Pilot Plant – Small scale fermenter to clarify optimum conditions

5. Industrial scale fermenter

Production of enzymes takes place in the following steps :

Isolation of Microorganisms, Strain Development and Preparation of Inoculum

Medium Formulation and Preparation

Sterilization and Inoculation of Medium, Maintenance of Culture and Fluid Filtration

Purification of Enzymes

gladiator answered 2 years ago

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