Question

What are three different ways to show that a specific protein (e.g., protein X) physically interacts with another specific protein (e.g., protein Y)?

Answer 1

Protein–protein interactions (PPIs) are the physical contacts of high specificity established between two or more protein molecules.

There are two main approaches for detecting interacting proteins:

1. IN VIVO METHODS: Yeast two-hybrid system

The most frequently used binary method is the yeast two-hybrid (Y2H) system. It has variations involving different reagents and has been adapted to high-throughput screening.

2. IN VITRO METHODS: IMMUNOPRECIPITATION(IP)/ Co-IP, and pull-down assay.

The most common co-complex method is coimmunoprecipitation (coIP).

Co-immunoprecipitation (co-IP) is a popular technique for protein interaction discovery.

Co-IP is conducted in essentially the same manner as an immunoprecipitation (IP) of a single protein,

For clear clarification, I am providing a table .

Source of article: Protein-Protein Interaction Detection: Methods and Analysis