Question

In: Biology

1. Which of the following transcription factor complexes is activated by Jak upon interferon (cytokine) activation?...

1. Which of the following transcription factor complexes is activated by Jak upon interferon (cytokine) activation?

a.

NF-kB

b.

Smads

c.

STATs

d.

c-Jun

e.

CREB

2. Interactions between intracellular signaling proteins are mediated by modular binding domains. Which of the following domain binds to a proline rich region?

a.

PH

b.

SH2

c.

PPP

d.

PTB

e.

SH3

3. Discussing PI 3-kinase: which of the following statements is correct?

a.	can directly phosphorylate phospholipase C-γ to produce DAG and IP3

b.	phosphorylates PI(4,5)P2 to produce PI(3,4,5)P3 that functions as docking sites for downstream proteins with a SH2 domain

c.	phosphorylates DAG to produce PI(3,4,5)P3 that functions as docking sites for downstream proteins with a SH3 domain

d.	phosphorylates PI(4,5)P2 to produce IP3 that functions as docking sites for downstream proteins with a PTB domain

e.	phosphorylates PI(4,5)P2 to produce PI(3,4,5)P3 that functions as docking sites for downstream proteins with a PH domain

Expert Solution

1.

a. NF-kB

explanation: NF-kB is not involved in JAK-STAT pathway. Hence option a is incorrect

b. Smads: smads is not onvolved JAK-STAT pathways. Hence option b is incorrect

c. STAT:

explanation: JAK (Janus Kinase family so called Non-tyrosine receptor kinase and STAT (for signal transducer and activator of transcription. Hence option c is correct.

d. c-Jun

explanation: Generally c-Jun combined with c-Fos form the AP-1 early response transcription factor. Hence option c is incorrect

d. CREB

explanation: it is cellular transcription factor. It binds to certain DNA sequences called cAMP response elements ( CRE), which leads to increasing or decreasing transcription of the downstream gene. Hence option d is incorrect

2.

a. PH

explanation: It recognize and bind phosphoinositides. Hence option a is incorrect.

b. SH2

explanation: SH2 domain that binds to P-Tyr. Hence option b is incorrect

c. PPP

explanation: This is domain is found in some members PPP phosphatase family, including serine/ threonine. Hence option c is incorrect

d. PTB

explanation: This domain recognize P-tyr. Hence option d is incorrect.

e. SH3

explanation: SH3 domain that binds proline rich regions in other proteins. Hence option e is correct.

3.

a. can directly phosphorylate phospholipase C- to produce DAG and IP3. Hence option a is incorrect

b. phosphorylates PI(4,5)P2 to produce PI (3,4,5) P3 that functions as docking sites for downstream proteins with a SH2 domain. Hence option b is incorrect

c. phosphorylates DAG to produce PI(3,4,5) P3 that functions as docking sites for downstream proteins with a SH3 domain. Hence option c is incorrect

d. phosphorylates PI(4,5) P2 to produce IP3 that functions as docking sites for downstream proteins with a PTB domain is correct statement. Hence option d is correct

e. phosphorylates PI(4,5) P2 to produce PI (3,4,5) P3 that functions as docking sites for downstream proteins with a PH domain in correct statement. Hence option e is incorrect.

gladiator answered 1 year ago

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