Question

Describe in your own words how any two types of the following types of chromatography work: adsorption, partition, ion exchange, molecular exclusion, or affinity.

Answer 1

Adsorption chromatography :basically chromatography is a method of separation.of two or more compounds. in adsorption chromatography is based n adsorption of the compound on the stationary phase.which is washed away by moving mobile phase. column chromatography is an example for the adsorption chromatography.in which mobile phase is silica gel or alumina which is closely packed and the compound to be separated is made adsorbed.depending on the functional group present in the compound the extent of adsorption will be different.an some compound will adhere strongly.some adsorb weakly and we can separate it easily by non polar solvent as mobile phase.as the mobile phase moves on the surface of the stationary phase in which the compound is attached.it losses the attachment with the stationary phase and comes out of the column first,but the compound which attached strongly need polar solvent to break the strong adsorption because of that it will come out of the column last.so the separation is effective.

Partition chromatography :in which the compounds to be separated is distributed between two liquids depending upon the partition coefficient of the material.If two compounds have different partition coefficient in two liquids say one mobile phase and another stationary phase then this method is effective in separation.one compound will be more soluble in one phase and less soluble in another phase,so separation is done.High performance liquid chromatography and gas chromatography is example for the partition chromatography.