Design an experiment to determine the changes in the transcriptome (global gene expression) of human cells...

Design an experiment to determine the changes in the transcriptome (global gene expression) of human cells in response to infection with the influenza virus. The experiment must be explained in detailed and have an appropriate control.

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gladiator answered 1 year ago

Design an experiment using RNA sequencing to determine the changes in the transcriptome (global gene expression)...

Design an experiment using RNA sequencing to determine the changes in the transcriptome (global gene expression) of human cells in response to infection with the influenza virus. For full credit, your experiment must be explained in detail and have an appropriate control.

You design a microarray experiment to determine the difference in gene expression patterns between normal and...

You design a microarray experiment to determine the difference in gene expression patterns between normal and cancerous lung tissue from humans. You label the cDNA derived from a lung tumor with a red fluorescent dye and the cDNA derived from normal lung tissue with a green fluorescent dye. You hybridize the cDNA to a microarray chip in which each spot corresponds to a DNA sequence from a different human gene. a. What can you infer about a particular gene’s expression...

Design an experiment to knock out a certain gene of interest in the human genome. Outline...

Design an experiment to knock out a certain gene of interest in the human genome. Outline the experiment, explaining the methods you would use, and also indicate how you would verify your experiment was successful at each step.

Explain the regulation of gene expression in eukaryotic cells. Discuss mechanisms by which gene expression may...

Explain the regulation of gene expression in eukaryotic cells. Discuss mechanisms by which gene expression may be altered. How do these alterations induce cancer-causing mutations in cell DNA? Explain how cancer is formed. Describe genetic changes found in cancer cells and how these changes lead to alterations in cell behavior. Determine whether proteome data can be utilized in genetic disorder diagnosis. Relate the Human Genome Project data to the analysis of cancer genes.

What does Global Gene Expression mean?

How does the 6S RNA promote global changes in gene expression? Provide examples of how small...

How does the 6S RNA promote global changes in gene expression? Provide examples of how small RNAs can promote RNA degradation? What is a molecular mechanism by which small RNAs promote translation? Provide an example where this may regulate a virulence factor. What is a molecular mechanism by which small RNAs inhibit translation? Provide an example where this may regulate a virulence factor. What is the function of Hfq and what role does it play in various mechanisms of post-transcriptional...

What is the advantage of using double negative 3 cells (DN3) cells to analyse gene expression...

What is the advantage of using double negative 3 cells (DN3) cells to analyse gene expression using real time PCR in leukemia research?

True or False: 1. In an individual human, the pattern of gene expression is identical across...

True or False: 1. In an individual human, the pattern of gene expression is identical across all different types of somatic cells 2. The stem cell potential of a fertilized human egg is pluripotent 3. In an individual human, different types of somatic cells have the same genome.

- - - - - design an experiment to determine the concentration of HbA1c in the...

- - - - - design an experiment to determine the concentration of HbA1c in the blood sample by following these six steps Step 1, pick one differentiating characteristic Step2 how will u probe that differentiating characteristic Step3 tell the anticipated response to the probe step4 measure the response to the probe step 5 how will u interpret the response to the probe to obtain the desired information step6 tell the likely interference with the method you have chosen. you...

The following drugs impair gene expression in cells. Outline the specific inhibitory mechanism for each. Erythromycin...

The following drugs impair gene expression in cells. Outline the specific inhibitory mechanism for each. Erythromycin Alpha-amanitin Tiamulin Tavaborole Flucytosine

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